Full metadata record
DC FieldValueLanguage
dc.creatorDricot, A. (Amélie)-
dc.creatorRual, J. J. (Jean François)-
dc.creatorLamesch, P. (Philippe)-
dc.creatorBertin, N. (Nicolas)-
dc.creatorDupuy, D. (Denis)-
dc.creatorHao, T. (Tong)-
dc.creatorLambert, C. (Christophe)-
dc.creatorHallez, R. (Régis)-
dc.creatorDelroisse, J.M. (Jean Marc)-
dc.creatorVandenhaute, J. (Jean)-
dc.creatorLopez-Goñi, I. (Ignacio)-
dc.creatorMoriyon, I. (Ignacio)-
dc.creatorGarcia-Lobo, J.M. (Juan María)-
dc.creatorSangari, F.J. (Félix Javier)-
dc.creatorMacMillan, A.P. (Alastair P.)-
dc.creatorCutler, S.J. (Sally J.)-
dc.creatorWhatmore, A.M. (Adrian M.)-
dc.creatorBozak, S. (Stephanie)-
dc.creatorSequerra, R. (Reynaldo)-
dc.creatorDoucettte-Stamm, L. (Lynn)-
dc.creatorVidal, M. (Marc)-
dc.creatorHill, D.E. (David E.)-
dc.creatorLetesson, J.J. (Jean Jacques)-
dc.creatorBolle, X. (Xavier) de-
dc.date.accessioned2013-07-01T10:14:05Z-
dc.date.available2013-07-01T10:14:05Z-
dc.date.issued2004-
dc.identifier.citationDricot A, Rual JF, Lamesch P, Bertin N, Dupuy D, Hao T, et al. Generation of the Brucella melitensis ORFeome version 1.1. Genome Res 2004 Oct;14(10B):2201-2206.es_ES
dc.identifier.issn1088-9051-
dc.identifier.urihttps://hdl.handle.net/10171/29445-
dc.description.abstractThe bacteria of the Brucella genus are responsible for a worldwide zoonosis called brucellosis. They belong to the alpha-proteobacteria group, as many other bacteria that live in close association with a eukaryotic host. Importantly, the Brucellae are mainly intracellular pathogens, and the molecular mechanisms of their virulence are still poorly understood. Using the complete genome sequence of Brucella melitensis, we generated a database of protein-coding open reading frames (ORFs) and constructed an ORFeome library of 3091 Gateway Entry clones, each containing a defined ORF. This first version of the Brucella ORFeome (v1.1) provides the coding sequences in a user-friendly format amenable to high-throughput functional genomic and proteomic experiments, as the ORFs are conveniently transferable from the Entry clones to various Expression vectors by recombinational cloning. The cloning of the Brucella ORFeome v1.1 should help to provide a better understanding of the molecular mechanisms of virulence, including the identification of bacterial protein-protein interactions, but also interactions between bacterial effectors and their host's targets.es_ES
dc.language.isoenges_ES
dc.publisherCold Spring Habor Laboratory Presses_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectBacterial proteins geneticses_ES
dc.subjectBrucella melitensis geneticses_ES
dc.subjectGenome, bacteriales_ES
dc.subjectOpen reading frames physiologyes_ES
dc.titleGeneration of the Brucella melitensis ORFeome version 1.1.es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.type.driverinfo:eu-repo/semantics/articlees_ES

Files in This Item:
Thumbnail
File
Generation of the Brucella.pdf
Description
Size
144.62 kB
Format
Adobe PDF


Statistics and impact
0 citas en
0 citas en

Items in Dadun are protected by copyright, with all rights reserved, unless otherwise indicated.