Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains

Lopez-Goñi, I. (Ignacio); Garcia-Yoldi, D. (David); Marin, C.M. (C. M.); Miguel, M.J. (María Jesús) de; Muñoz, P. (Pilar); Blasco, J.M. (J. M.); Jacques, I. (I.); Grayon, M. (M.); Cloeckaert, A. (Axel); Ferreira, A.C. (A. C.); Cardoso, R. (R.); Correa-de-Sa, M.I. (M. I.); Walravens, K. (K.); Albert, D. (D.); Garin-Bastuji, B. (B.)

Keywords:

Abortus
Melitensis
Differentiation
Identification
RB51
Hybridization
Deletion
Genes
Suis
S19

Issue Date:

2008

Publisher:

American Society for Microbiology

ISSN:

0095-1137

Citation:

Lopez-Goni I, Garcia-Yoldi D, Marin CM, de Miguel MJ, Munoz PM, Blasco JM, et al. Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains. J Clin Microbiol 2008 Oct;46(10):3484-3487.

Abstract

An evaluation of a multiplex PCR assay (Bruce-ladder) was performed in seven laboratories using 625 Brucella strains from different animal and geographical origins. This robust test can differentiate in a single step all of the classical Brucella species, including those found in marine mammals and the S19, RB51, and Rev.1 vaccine strains.

URI:

https://hdl.handle.net/10171/29507

Appears in Collections:

DA - Medicina - Microbiología y Parasitología - Artículos de revista

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