Performance of competitive and indirect enzyme-linked immunosorbent assays, gel immunoprecipitation with native hapten polysaccharide, and standard serological tests in diagnosis of sheep brucellosis

Marin, C.M. (C. M.); Moreno, E. (Edgardo); Moriyon, I. (Ignacio); Diaz, R. (Ramón); Blasco, J.M. (J. M.)

Authors:

Marin, C.M. (C. M.)

Moreno, E. (Edgardo)

Moriyon, I. (Ignacio)

Diaz, R. (Ramón)

Blasco, J.M. (J. M.)

Keywords:

Brucella melitensis
Brucellosis diagnosis
Enzyme-Linked immunosorbent assay methods
Haptens analysis
Sheep diseases diagnosis

Issue Date:

1999

Publisher:

American Society for Microbiology

ISSN:

1071-412x

Citation:

Marin CM, Moreno E, Moriyon I, Diaz R, Blasco JM. Performance of competitive and indirect enzyme-linked immunosorbent assays, gel immunoprecipitation with native hapten polysaccharide, and standard serological tests in diagnosis of sheep brucellosis. Clin Diagn Lab Immunol 1999 Mar;6(2):269-272.

Abstract

Competitive and standard enzyme-linked immunosorbent assays (ELISAs), rose bengal (RB), complement fixation, and agar gel immunoprecipitation with native hapten (AGID-NH) were compared by using sera from Brucella-free, Brucella melitensis-infected, and B. melitensis Rev1-vaccinated sheep. The most sensitive tests were indirect ELISA and RB, and the most specific tests were AGID-NH and competitive ELISA. We show that RB followed by AGID-NH is a simple and effective system for diagnosing sheep brucellosis.

URI:

https://hdl.handle.net/10171/29516

Appears in Collections:

DA - Medicina - Microbiología y Parasitología - Artículos de revista

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