Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR

Romero, C. (Conchi); Lopez-Goñi, I. (Ignacio)

Authors:

Romero, C. (Conchi)

Lopez-Goñi, I. (Ignacio)

Keywords:

Blood
RNA
Biotechnology and applied microbiology
Microbiology

Issue Date:

1999

Publisher:

American Society for Microbiology

ISSN:

0099-2240

Citation:

Romero C, Lopez-Goni I. Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR. Appl Environ Microbiol 1999 Aug;65(8):3735-3737.

Abstract

Different methods of extraction of bacterial DNA from bovine milk to improve the direct detection of Brucella by PCR were evaluated. We found that the use of a lysis buffer with high concentrations of Tris, EDTA, and NaCl, high concentrations of sodium dodecyl sulfate and proteinase K, and high temperatures of incubation was necessary for the efficient extraction of Brucella DNA. The limit of detection by PCR was 5 to 50 Brucella CFU/ml of milk.

URI:

https://hdl.handle.net/10171/29637

Appears in Collections:

DA - Medicina - Microbiología y Parasitología - Artículos de revista

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