Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Abajo, A. (Ana) | - |
dc.creator | Bitarte, N. (Nerea) | - |
dc.creator | Zarate, R. (Ruth) | - |
dc.creator | Boni, V. (Valentina) | - |
dc.creator | Lopez, I. (Inés) | - |
dc.creator | Gonzalez-Huarriz, M. (Marisol) | - |
dc.creator | Rodriguez, J. (Javier) | - |
dc.creator | Bandres, E. (Eva) | - |
dc.creator | Garcia-Foncillas, J. (Jesús) | - |
dc.date.accessioned | 2014-07-15T11:29:26Z | - |
dc.date.available | 2014-07-15T11:29:26Z | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | Abajo A, Bitarte N, Zarate R, Boni V, Lopez I, Gonzalez-Huarriz M, et al. Identification of colorectal cancer metastasis markers by an angiogenesis-related cytokine-antibody array. World J Gastroenterol. 2012 Feb 21;18(7):637-45. | es_ES |
dc.identifier.issn | 1007-9327 | - |
dc.identifier.uri | https://hdl.handle.net/10171/36165 | - |
dc.description.abstract | AIM: To investigate the angiogenesis-related protein expression profile characterizing metastatic colorectal cancer (mCRC) with the aim of identifying prognostic markers. METHODS: The expression of 44 angiogenesis-secreted factors was measured by a novel cytokine antibody array methodology. The study evaluated vascular endothelial growth factor (VEGF) and its soluble vascular endothelial growth factor receptor (sVEGFR)-1 protein levels by enzyme immunoassay (EIA) in a panel of 16 CRC cell lines. mRNA VEGF and VEGF-A isoforms were quantified by quantitative reverse-transcription polymerase chain reaction (Q-RT-PCR) and vascular endothelial growth factor receptor (VEGFR)-2 expression was analyzed by flow cytometry. RESULTS: Metastasis-derived CRC cell lines expressed a distinctive molecular profile as compared with those isolated from a primary tumor site. Metastatic CRC cell lines were characterized by higher expression of angiogenin-2 (Ang-2), macrophage chemoattractant proteins-3/4 (MCP-3/4), matrix metalloproteinase-1 (MMP-1), and the chemokines interferon γ inducible T cell α chemoattractant protein (I-TAC), monocyte chemoattractant protein I-309, and interleukins interleukin (IL)-2 and IL-1α, as compared to primary tumor cell lines. In contrast, primary CRC cell lines expressed higher levels of interferon γ (IFN-γ), insulin-like growth factor-1 (IGF-1), IL-6, leptin, epidermal growth factor (EGF), placental growth factor (PlGF), thrombopoietin, transforming growth factor β1 (TGF-β1) and VEGF-D, as compared with the metastatic cell lines. VEGF expression does not significantly differ according to the CRC cellular origin in normoxia. Severe hypoxia induced VEGF expression up-regulation but contrary to expectations, metastatic CRC cell lines did not respond as much as primary cell lines to the hypoxic stimulus. In CRC primary-derived cell lines, we observed a two-fold increase in VEGF expression between normoxia and hypoxia as compared to metastatic cell lines. CRC cell lines express a similar pattern of VEGF isoforms (VEGF₁₂₁, VEGF₁₆₅ and VEGF₁₈₉) despite variability in VEGF expression, where the major transcript was VEGF₁₂₁. No relevant expression of VEGFR-2 was found in CRC cell lines, as compared to that of human umbilical vein endothelial cells and sVEGFR-1 expression did not depend on the CRC cellular origin. CONCLUSION: A distinct angiogenesis-related expression pattern characterizes metastatic CRC cell lines. Factors other than VEGF appear as prognostic markers and intervention targets in the metastatic CRC setting. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI AG | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Colorectal cancer metastasis | es_ES |
dc.subject | Cytokine-antibody array | es_ES |
dc.subject | Angiogenesis | es_ES |
dc.subject | Vascular endothelial growth factor | es_ES |
dc.subject | Biomarkers | es_ES |
dc.title | Identification of colorectal cancer metastasis markers by an angiogenesis-related cytokine-antibody array | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | http://dx.doi.org/10.3748/wjg.v18.i7.637. | es_ES |
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