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dc.creatorFernandez-Robredo, P. (Patricia)-
dc.creatorRecalde, S. (Sergio)-
dc.creatorMoreno-Orduña, M. (Maite)-
dc.creatorGarcia-Garcia, L. (Laura)-
dc.creatorZarranz-Ventura, J. (Javier)-
dc.creatorGarcia-Layana, A. (Alfredo)-
dc.date.accessioned2014-08-23T10:58:17Z-
dc.date.available2014-08-23T10:58:17Z-
dc.date.issued2013-
dc.identifier.citationFernández-Robredo P, Recalde S, Moreno-Orduña M, García-García L, Zarranz-Ventura J, García-Layana A. Azithromycin reduces inflammation in a rat model of acute conjunctivitis. Mol Vis. 2013;19:153-165es_ES
dc.identifier.issn1090-0535-
dc.identifier.urihttp://hdl.handle.net/10171/36369-
dc.description.abstractPURPOSE: Macrolide antibiotics are known to have various anti-inflammatory effects in addition to their antimicrobial activity, but the mechanisms are still unclear. The effect of azithromycin on inflammatory molecules in the lipopolysaccharide-induced rat conjunctivitis model was investigated. METHODS: Twenty-four Wistar rats were divided into two groups receiving topical ocular azithromycin (15 mg/g) or vehicle. In total, six doses (25 µl) were administered as one dose twice a day for three days before subconjunctival lipopolysaccharide injection (3 mg/ml). Before the rats were euthanized, mucus secretion, conjunctival and palpebral edema and redness were evaluated. Real-time polymerase chain reaction was used to determine gene expression for interleukin-6, cyclooxygenase-2, tumor necrosis factor-α, matrix metalloproteinase (MMP)-2, and MMP-9. Interleukin-6 was determined with enzyme-linked immunosorbent assay, nuclear factor-kappa B with western blot, and MMP-2 activity with gelatin zymogram. Four eyes per group were processed for histology and subsequent periodic acid-Schiff staining and CD68 for immunofluorescence. The Student t test or the Wilcoxon test for independent samples was applied (SPSS v.15.0). RESULTS: Azithromycin-treated animals showed a significant reduction in all clinical signs (p<0.05) compared to controls. Interleukin-6 (p<0.05), nuclear factor-kappa B protein expression (p<0.01), and MMP-2 activity (p<0.05) in conjunctival homogenates were significantly reduced compared with the control animals. MMP-2 gene expression showed a tendency to decrease in the azithromycin group (p=0.063). Mucus secretion by goblet cells and the macrophage count in conjunctival tissue were also decreased in the azithromycin group (p<0.05). CONCLUSIONS: These results suggest that azithromycin administration ameliorates induced inflammation effects in a rat model of acute conjunctivitis.es_ES
dc.language.isoenges_ES
dc.publisherMolecular Visiones_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectAzithromycines_ES
dc.subjectRatses_ES
dc.subjectConjunctivitises_ES
dc.subjectAnti-Inflammatory Agents, Non-Steroidales_ES
dc.titleAzithromycin reduces inflammation in a rat model of acute conjunctivitises_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://www.molvis.org/molvis/v19/153/es_ES

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