Innervation of a prefabricated flap: a new experimental model
Palabras clave : 
Flap
Innervation
Neoaxonogenesis
Experimental model
Fecha de publicación: 
2014
Editorial : 
Hindawi Publishing Corporation
ISSN: 
2314-6133
Cita: 
Romeo M, Cuccia G, Qiu SS, Raimondo S, Geuna S, Hontanilla B. Innervation of a prefabricated flap: a new experimental model. Biomed Res Int. 2014;2014:549819.
Resumen
Introduction. Flap innervation by neoaxonogenesis is a promising field of investigation. The authors evaluated the possibility of innervating an acellular collagen scaffold as component of a potential prefabricated flap. Materials and Methods. Collagen matrix sheets were implanted around the femoral bundle of a murine model to produce two flaps on proximal and distal nerve stumps based on a flow-through model. After thirty days, nerve regeneration and integration into the collagen matrix were evaluated. The specimens were microscopically analyzed to study Schwann cell colonization and axonal integration with the matrix. Axonal count and density were assessed and statistically evaluated. Results. Qualitative structural and ultrastructural evaluation indicated integration, with axonal fibers merged within the collagen matrix, along with a newly formed vascular network on the proximal flap. Wallerian degeneration occurred inside the distal chamber. Axonal count and density did not show statistically significant differences between the nerve inside the proximal flap and the control side. Conclusions. Innervation of an acellular matrix can be obtained by direct nerve stump implantation. The flow-through system was relatively easy to build and reliable to provide adequate blood supply. The collagen scaffold may be a promising support or further studies of preinnervated microsurgical flaps.

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Fichero: 
BMRI2014-549819.pdf
Descripción: 
Tamaño: 
11,06 MB
Formato: 
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