Validation of a UHPLC-FLD method for the simultaneous quantification of aflatoxins, ochratoxin A and zearalenone in barley
Keywords: 
Mycotoxin.
Aflatoxins
Ochratoxin A
Zearalenone
UHPLC-FLD
Barley
Issue Date: 
2011
Publisher: 
Elsevier
ISSN: 
0308-8146
Citation: 
Ibáñez-Vea M, Corcuera LA, Murillo-Arbizu MT, González-Peñas E, Lizarraga E. Validation of a UHPLC-FLD method for the simultaneous quantification of aflatoxins, ochratoxin A and zearalenone in barley. Food Chem 2011 Jul;127(1):351–358
Abstract
A fast and simple UHPLC-FLD method has been developed for the simultaneous determination in barley of aflatoxins (B1, G1, B2 and G2), ochratoxin A (OTA) and zearalenone (ZEA), some of the most important mycotoxins due to their toxicity and occurrence. The procedure is based on the extraction of the six mycotoxins with a mixture of acetonitrile and water, and the purification of the extract with immunoaffinity columns before analysis. Detection of AFB1 and AFG1 is improved using a photochemical reaction. The method has been validated with satisfactory results. Limits of detection were 340 ng kg-1 for ZEA, 13 ng kg-1 for OTA and varied from 0.5 to 15 ng kg-1 for aflatoxins. Recovery percentages were between 78.2 and 109.2%. After being validated, the method has been successfully applied to 20 barley samples cultivated in a region of northern Spain (Navarra).

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