Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Paino, T. (Teresa) | - |
dc.creator | Sarasquete, M.E. (María E.) | - |
dc.creator | Paiva, B. (Bruno) | - |
dc.creator | Krzeminski, P. (Patryk) | - |
dc.creator | San-Segundo, L. (Laura) | - |
dc.creator | Corchete, L.A. (Luis A.) | - |
dc.creator | Redondo, A. M. (Alba M.) | - |
dc.creator | Garayoa, M. (Mercedes) | - |
dc.creator | García-Sanz, R. (Ramón) | - |
dc.creator | Gutierrez, N.C. (Norma C.) | - |
dc.creator | Ocio, E.M. (Enrique M.) | - |
dc.creator | San-Miguel, J.F. (Jesús F.) | - |
dc.date.accessioned | 2015-05-11T10:55:43Z | - |
dc.date.available | 2015-05-11T10:55:43Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Paíno T, Sarasquete ME, Paiva B, Krzeminski P, San-Segundo L, Corchete LA, et al. Phenotypic, genomic and functional characterization reveals no differences between CD138++ and CD138low subpopulations in multiple myeloma cell lines. PLoS One. 2014 ;9(3):e92378. | es_ES |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://hdl.handle.net/10171/38274 | - |
dc.description.abstract | Despite recent advances in the treatment of multiple myeloma (MM), it remains an incurable disease potentially due to the presence of resistant myeloma cancer stem cells (MM-CSC). Although the presence of clonogenic cells in MM was described three decades ago, the phenotype of MM-CSC is still controversial, especially with respect to the expression of syndecan-1 (CD138). Here, we demonstrate the presence of two subpopulations--CD138++ (95-99%) and CD138low (1-5%)--in eight MM cell lines. To find out possible stem-cell-like features, we have phenotypically, genomic and functionally characterized the two subpopulations. Our results show that the minor CD138low subpopulation is morphologically identical to the CD138++ fraction and does not represent a more immature B-cell compartment (with lack of CD19, CD20 and CD27 expression). Moreover, both subpopulations have similar gene expression and genomic profiles. Importantly, both CD138++ and CD138low subpopulations have similar sensitivity to bortezomib, melphalan and doxorubicin. Finally, serial engraftment in CB17-SCID mice shows that CD138++ as well as CD138low cells have self-renewal potential and they are phenotypically interconvertible. Overall, our results differ from previously published data in MM cell lines which attribute a B-cell phenotype to MM-CSC. Future characterization of clonal plasma cell subpopulations in MM patients' samples will guarantee the discovery of more reliable markers able to discriminate true clonogenic myeloma cells. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library of Science | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Phenotypic | es_ES |
dc.subject | Multiple myeloma | es_ES |
dc.subject | MM-CSC | es_ES |
dc.title | Phenotypic, genomic and functional characterization reveals no differences between CD138++ and CD138low subpopulations in multiple myeloma cell lines | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | http://dx.doi.org/ 10.1371/journal.pone.0092378 | es_ES |
Files in This Item:
Statistics and impact
Items in Dadun are protected by copyright, with all rights reserved, unless otherwise indicated.