Generation and characterization of human iPSC line generated from mesenchymal stem cells derived from adipose tissue
Palabras clave : 
Materias Investigacion::Ciencias de la Salud::Microbiología y biología molecular
Materias Investigacion::Ciencias de la Salud::Oncología
Mesenchymal stem cells
Adipose tissue
Human iPSC line
Stem cell
Fecha de publicación: 
2016
Editorial : 
Elsevier
ISSN: 
1873-5061
Nota: 
An open access article under the CC BY-NC-ND license
Cita: 
Zapata-Linares NM, Rodríguez S, Mazo M, Abizanda G, Andreu EJ, Barajas M, et al. Generation and characterization of human iPSC line generated from mesenchymal stem cells derived from adipose tissue. Stem Cell Res 2016;16(1):20–23
Resumen
Abstract In this work, mesenchymal stem cells derived from adipose tissue (ADSCs) were used for the generation of the human-induced pluripotent stem cell line G15.AO. Cell reprogramming was performed using retroviral vectors containing the Yamanaka factors, and the generated G15.AO hiPSC line showed normal karyotype, silencing of the exogenous reprogramming factors, induction of the typical pluripotency-associated markers, alkaline phosphatase enzymatic activity, and in vivo and in vitro differentiation ability to the three germ layers.

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1-s2.0-S1873506115001725-main.pdf
Descripción: 
Tamaño: 
1,21 MB
Formato: 
Adobe PDF


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