Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Gonzalez-Peñas, E. (Elena) | - |
dc.creator | Flores-Flores, M.E. (Myra Evelyn) | - |
dc.date.accessioned | 2016-03-01T13:12:46Z | - |
dc.date.available | 2016-03-01T13:12:46Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Flores-Flores ME, González-Peñas E. Development and validation of a high performance liquid chromatographic-mass spectrometry method for the simultaneous quantification of 10 trichothecenes in ultra-high temperature processed cow milk. J Chromatogr A. 2015;1419:37-44 | es_ES |
dc.identifier.issn | 0021-9673 | - |
dc.identifier.uri | https://hdl.handle.net/10171/40092 | - |
dc.description.abstract | An LC-MS/MS (QqQ) method has been developed and validated for simultaneous determination of the following trichothecenes in UHT cow milk: nivalenol (NIV), deoxynivalenol (DON), deepoxy-deoxynivalenol (DOM-1), 3-acetyldeoxynivalenol (3- ADON), 15-acetyldeoxynivalenol (15-ADON), neosolaniol (NEO), diacetoxyscirpenol (DAS), fusarenon X (FUS-X), T-2 and HT-2 toxins. Sample treatment is simple and based on the extraction with acetonitrile (ACN), acidified with 0.2% formic acid, followed by a purification process, adding sodium acetate to the ACN/water extract in order to separate aqueous phase and, consequently, polar components of the milk. Validation of the method for all the 10 mycotoxins was successful; validation parameters taken into account were as follows: limits of detection (LOD) and quantification (LOQ), linearity, precision (within-day and between-day variability), recovery, matrix effect and stability. The LODs were 10.1, 2.5, 1.5, 1.9, 0.1, 0.5, 1.0, 0.08, 0.4 and 0.05 ng/mL for NIV, DON, DOM-1, FUS-X, NEO, 3-ADON, 15-ADON, DAS, HT-2 and T-2, respectively. Mean recovery values (obtained in intermediate precision conditions) were between 63.5 and 75.8 (RSDR ≤ 15%) for all the mycotoxins. All the mycotoxins suffered from matrix effects, especially DON. | es_ES |
dc.description.sponsorship | Programa de Investigación Universidad de Navarra (PIUNA) | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | * |
dc.subject | LC-MS/MS | es_ES |
dc.subject | UHT cow milk | es_ES |
dc.subject | Milk | es_ES |
dc.subject | Trichothecenes | es_ES |
dc.subject | Mycotoxin | es_ES |
dc.title | Development and validation of a high performance liquid chromatographic-mass spectrometry method for the simultaneous quantification of 10 trichothecenes in ultra-high temperature processed cow milk | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | http://dx.doi.org/10.1016/j.chroma.2015.09.069 | es_ES |
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