Acanda-de-la-Rocha, A.M. (Arlet María); Lopez-Bertoni, H. (Hernando); Guruceaga, E. (Elizabeth); et al. "Analysis of SOX2-regulated transcriptome in glioma stem cells". PlosOne. 11 (9), 2016,1-20
Glioblastoma is the most malignant brain tumor in adults and is associated with poor sur-
vival despite multimodal treatments. Glioma stem-like cells (GSCs) are cells functionally
defined by their self-renewal potential and the ability to reconstitute the original tumor upon
orthotopic implantation. They have been postulated to be the culprit of glioma chemo- and
radio-resistance ultimately leading to relapse. Understanding the molecular circuits govern-
ing the GSC compartment is essential. SOX2, a critical transcription regulator of embryonic
and neural stem cell function, is deregulated in GSCs however; the precise molecular path-
ways regulated by this gene in GSCs remain poorly understood.
We performed a genome-wide analysis of SOX2-regulated transcripts in GSCs, using a
microarray. We identified a total of 2048 differentially expressed coding transcripts and 261
non-coding transcripts. Cell adhesion and cell-cell signaling are among the most enriched
terms using Gene Ontology (GO) classification. The pathways altered after SOX2 down-
modulation includes multiple cellular processes such as amino-acid metabolism and inter-
cellular signaling cascades. We also defined and classified the set of non-coding transcripts
differentially expressed regulated by SOX2 in GSCs, and validated two of them.
We present a comprehensive analysis of the transcriptome controlled by SOX2 in GSCs,
gaining insights in the understanding of the potential roles of SOX2 in glioblastoma.