Flores-Flores, M.E. (Myra Evelyn); Gonzalez-Peñas, E. (Elena). "An LC-MS/MS method for multi-mycotoxin quantification in cow milk". Food Chem , 2017-09, 378 - 385
The simultaneous quantification of 15 mycotoxins in cow milk by liquid
chromatography-mass spectrometry, is presented. Extraction was performed with
acidified acetonitrile, followed by a cleanup step with sodium acetate. During validation
limits of detection (LOD) and quantification, linearity, precision, accuracy, recovery,
matrix effect, and stability were studied. LOD values were between 0.02 and 10.14
ng/mL for aflatoxins M1, B1, B2, G1, G2, ochratoxins A and B, HT-2 and T-2 toxins,
deepoxy-deoxynivalenol, zearalenone, sterigmatocystin and fumonisins B1, B2 and B3.
Recovery values were between 82.6 and 94.4% for all the mycotoxins, except for
fumonisins. The recovery values for fumonisins were between 42.1% and 64.6%.
Matrix effect, between 25.5 and 96.8%, appeared for all of the mycotoxins, especially
for deepoxy-deoxynivalenol, zearalenone and sterigmatocystin. The validated method
achieves the quantification of those mycotoxins of major concern and mycotoxins that
are not frequently studied in milk, such as fumonisins, sterigmatocystin or ochratoxin B.