Full metadata record
DC Field | Value | Language |
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dc.creator | Llopiz, D. (Diana) | - |
dc.creator | Ruiz, M. (Marta) | - |
dc.creator | Infante, S. (Stefany) | - |
dc.creator | Villanueva, L. (Lorea) | - |
dc.creator | Silva, L. (Leyre) | - |
dc.creator | Hervas-Stubbs, S. (Sandra) | - |
dc.creator | Alignani, D. (Diego) | - |
dc.creator | Guruceaga, E. (Elizabeth) | - |
dc.creator | Lasarte, J.J. (Juan José) | - |
dc.creator | Sarobe, P. (Pablo) | - |
dc.date.accessioned | 2019-04-01T12:31:46Z | - |
dc.date.available | 2019-04-01T12:31:46Z | - |
dc.date.issued | 2016-01-01 | - |
dc.identifier.citation | Llopiz, D. (Diana); Ruiz, M. (Marta); Infante, S. (Stefany); et al. "IL-10 expression defines an immunosuppressive dendritic cell population induced by antitumor therapeutic vaccination". Oncotarget. 8 (2), 2016-01-01, 2659 - 2671 | es |
dc.identifier.uri | https://hdl.handle.net/10171/56665 | - |
dc.description.abstract | Vaccination induces immunostimulatory signals that are often accompanied by regulatory mechanisms such as IL-10, which control T-cell activation and inhibit vaccine-dependent antitumor therapeutic effect. Here we characterized IL- 10-producing cells in different tumor models treated with therapeutic vaccines. Although several cell subsets produced IL-10 irrespective of treatment, an early vaccine-dependent induction of IL-10 was detected in dendritic cells (DC). IL-10 production defined a DC population characterized by a poorly mature phenotype, lower expression of T-cell stimulating molecules and upregulation of PD-L1. These IL-10+ DC showed impaired in vitro T-cell stimulatory capacity, which was rescued by incubation with IL-10R and PD-L1-inhibiting antibodies. In vivo IL-10 blockade during vaccination decreased the proportion of IL-10+ DC and improved their maturation, without modifying PD-L1 expression. Similarly, PD-L1 blockade did not affect IL-10 expression. Interestingly, vaccination combined with simultaneous blockade of IL-10 and PD-L1 induced stronger immune responses, resulting in a higher therapeutic efficacy in tumor-bearing mice. These results show that vaccine-induced immunoregulatory IL-10+ DC impair priming of antitumor immunity, suggesting that therapeutic vaccination protocols may benefit from combined targeting of inhibitory molecules expressed by this DC subset. | es_ES |
dc.description.sponsorship | This work was supported by Ministerio de Economia y Competitividad/Instituto de Salud Carlos III (grant PI14/00343) to PS. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Impact Journals | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Dendritic cells | es_ES |
dc.subject | Therapeutic vaccination | es_ES |
dc.subject | IL-10 | es_ES |
dc.subject | PD-L1 | es_ES |
dc.subject | Immunoregulation | es_ES |
dc.title | IL-10 expression defines an immunosuppressive dendritic cell population induced by antitumor therapeutic vaccination | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.description.note | Creative Commons Attribution 3.0 License. | es_ES |
dc.identifier.doi | https://doi.org/10.18632/oncotarget.13736 | es_ES |
dc.identifier.doi | https://doi.org/10.18632/oncotarget.13736 | - |
dadun.citation.endingPage | 2671 | es_ES |
dadun.citation.number | 2 | es_ES |
dadun.citation.publicationName | Oncotarget | es_ES |
dadun.citation.startingPage | 2659 | es_ES |
dadun.citation.volume | 8 | es_ES |
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