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dc.creatorLlopiz, D. (Diana)-
dc.creatorRuiz, M. (Marta)-
dc.creatorInfante, S. (Stefany)-
dc.creatorVillanueva, L. (Lorea)-
dc.creatorSilva, L. (Leyre)-
dc.creatorHerva-Stubbs, S. (Sandra)-
dc.creatorAlignani, D. (Diego)-
dc.creatorGuruceaga, E. (Elizabeth)-
dc.creatorLasarte, J.J. (Juan José)-
dc.creatorSarobe, P. (Pablo)-
dc.date.accessioned2019-04-01T12:31:46Z-
dc.date.available2019-04-01T12:31:46Z-
dc.date.issued2016-01-01-
dc.identifier.citationLlopiz, D. (Diana); Ruiz, M. (Marta); Infante, S. (Stefany); et al. "IL-10 expression defines an immunosuppressive dendritic cell population induced by antitumor therapeutic vaccination". Oncotarget. 8 (2), 2016-01-01, 2659 - 2671es
dc.identifier.urihttp://hdl.handle.net/10171/56665-
dc.description.abstractVaccination induces immunostimulatory signals that are often accompanied by regulatory mechanisms such as IL-10, which control T-cell activation and inhibit vaccine-dependent antitumor therapeutic effect. Here we characterized IL- 10-producing cells in different tumor models treated with therapeutic vaccines. Although several cell subsets produced IL-10 irrespective of treatment, an early vaccine-dependent induction of IL-10 was detected in dendritic cells (DC). IL-10 production defined a DC population characterized by a poorly mature phenotype, lower expression of T-cell stimulating molecules and upregulation of PD-L1. These IL-10+ DC showed impaired in vitro T-cell stimulatory capacity, which was rescued by incubation with IL-10R and PD-L1-inhibiting antibodies. In vivo IL-10 blockade during vaccination decreased the proportion of IL-10+ DC and improved their maturation, without modifying PD-L1 expression. Similarly, PD-L1 blockade did not affect IL-10 expression. Interestingly, vaccination combined with simultaneous blockade of IL-10 and PD-L1 induced stronger immune responses, resulting in a higher therapeutic efficacy in tumor-bearing mice. These results show that vaccine-induced immunoregulatory IL-10+ DC impair priming of antitumor immunity, suggesting that therapeutic vaccination protocols may benefit from combined targeting of inhibitory molecules expressed by this DC subset.es_ES
dc.description.sponsorshipThis work was supported by Ministerio de Economia y Competitividad/Instituto de Salud Carlos III (grant PI14/00343) to PS.es_ES
dc.language.isoenges_ES
dc.publisherImpact Journalses_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectDendritic cellses_ES
dc.subjectTherapeutic vaccinationes_ES
dc.subjectIL-10es_ES
dc.subjectPD-L1es_ES
dc.subjectImmunoregulationes_ES
dc.titleIL-10 expression defines an immunosuppressive dendritic cell population induced by antitumor therapeutic vaccinationes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttps://doi.org/10.18632/oncotarget.13736es_ES
dc.description.noteCreative Commons Attribution 3.0 License.es_ES
dc.identifier.doihttps://doi.org/10.18632/oncotarget.13736-
dadun.citation.endingPage2671es_ES
dadun.citation.number2es_ES
dadun.citation.publicationNameOncotargetes_ES
dadun.citation.startingPage2659es_ES
dadun.citation.volume8es_ES

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