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dc.creatorArce-López, B. (Beatriz)-
dc.creatorLizarraga, E. (Elena)-
dc.creatorFlores-Flores, M.E. (Myra Evelyn)-
dc.creatorIrigoyen, A. (Angel)-
dc.creatorGonzalez-Peñas, E. (Elena)-
dc.date.accessioned2019-09-05T07:55:31Z-
dc.date.available2019-09-05T07:55:31Z-
dc.date.issued2020-
dc.identifier.citationArce-López, B. (Beatriz); Lizarraga, E. (Elena); Flores-Flores, M.E. (Myra Evelyn); et al. "Development and validation of a methodology based on Captiva EMR-lipid clean-up and LC-MS/MS analysis for the simultaneous determination of mycotoxins in human plasma". Talanta. 206, 2020, 120193es
dc.identifier.issn0039-9140-
dc.identifier.urihttp://hdl.handle.net/10171/58063-
dc.description.abstractWe report the methodology for the quantification of 19 mycotoxins in human plasma using high performance liquid chromatography-mass spectrometry (triple quadrupole). The studied mycotoxins were: deepoxy-deoxynivalenol, aflatoxins (B1, B2, G1, G2 and M1), T-2 and HT-2, ochratoxins A and B, zearalenone, sterigmatocystin, nivalenol, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, neosolaniol, diacetoxyscirpenol and fusarenon-X. Sample deproteinization and cleanup were performed in one step using Captiva EMR-lipid (3 mL) cartridges and acetonitrile (with 1% formic acid). The extraction step was simple and fast. Validation was based on the evaluation of limits of detection (LOD) and quantification, linearity, precision, recovery, matrix effect, and stability. LOD values ranged from 0.04 ng/mL for aflatoxin B1 to 2.7 ng/mL for HT-2, except for nivalenol, which was 9.1 ng/mL. Recovery was obtained in intermediate precision conditions and at three concentration levels. Mean values ranged from 68.8% for sterigmatocystin to 97.6% for diacetoxyscirpenol (RDS ≤ 15% for all the mycotoxins). Matrix effects (assessed at three concentration levels and in intermediate conditions) were not significant for most of the mycotoxins and were between 75.4% for sterigmatocystin and 109.3% for ochratoxin B (RDS ≤ 15% for all the mycotoxins). This methodology will be useful in human biomonitoring studies of mycotoxins for its reliability.es_ES
dc.description.sponsorshipThis work was supported by the Spanish “Ministerio de Economía, Industria y Competitividad, Agencia Estatal de Investigación” (AGL2017-85732-R) (MINECO/AEI/FEDER, UE).es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsinfo:eu-repo/semantics/embargoedAccesses_ES
dc.subjectMycotoxinses_ES
dc.subjectMulti-detectiones_ES
dc.subjectHuman plasmaes_ES
dc.subjectLC-MS/MSes_ES
dc.subjectHuman biomonitoringes_ES
dc.titleDevelopment and validation of a methodology based on Captiva EMR-lipid clean-up and LC-MS/MS analysis for the simultaneous determination of mycotoxins in human plasmaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.talanta.2019.120193es_ES
dc.description.noteCreative Commons Attribution Non-Commercial No Derivatives Licensees_ES
dc.identifier.doihttps://doi.org/10.1016/j.talanta.2019.120193-
dadun.citation.publicationNameTalantaes_ES
dadun.citation.startingPage120193es_ES
dadun.citation.volume206es_ES

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