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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.creator | Martínez-Negro, M. (María) | - |
dc.creator | Sánchez-Arribas, N. (Natalia) | - |
dc.creator | Guerrero-Martínez, A. (Andrés) | - |
dc.creator | Moyá, M.L. (María Luisa) | - |
dc.creator | Tros-de-Ilarduya, C. (Conchita) | - |
dc.creator | Mendicuti, F. (Francisco) | - |
dc.creator | Aicart, E. (Emilio) | - |
dc.creator | Junquera, E. (Elena) | - |
dc.date.accessioned | 2021-09-09T11:47:56Z | - |
dc.date.available | 2021-09-09T11:47:56Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Martínez-Negro, M. (María); Sánchez-Arribas, N. (Natalia); Guerrero-Martínez, A. (Andrés); et al. "A Non-Viral Plasmid DNA Delivery System Consisting on a Lysine-Derived Cationic Lipid Mixed with a Fusogenic Lipid". Pharmaceutics. 11 (632), 2019, 1 - 16 | es_ES |
dc.identifier.issn | 1999-4923 | - |
dc.identifier.other | PMID: 31783620 | - |
dc.identifier.uri | https://hdl.handle.net/10171/61973 | - |
dc.description.abstract | The insertion of biocompatible amino acid moieties in non-viral gene nanocarriers is an attractive approach that has been recently gaining interest. In this work, a cationic lipid, consisting of a lysine-derived moiety linked to a C12 chain (LYCl) was combined with a common fusogenic helper lipid (DOPE) and evaluated as a potential vehicle to transfect two plasmid DNAs (encoding green fluorescent protein GFP and luciferase) into COS-7 cells. A multidisciplinary approach has been followed: (i) biophysical characterization based on zeta potential, gel electrophoresis, small-angle X-ray scattering (SAXS), and cryo-transmission electronic microscopy (cryo-TEM); (ii) biological studies by fluorescence assisted cell sorting (FACS), luminometry, and cytotoxicity experiments; and (iii) a computational study of the formation of lipid bilayers and their subsequent stabilization with DNA. The results indicate that LYCl/DOPE nanocarriers are capable of compacting the pDNAs and protecting them efficiently against DNase I degradation, by forming Lα lyotropic liquid crystal phases, with an average size of ~200 nm and low polydispersity that facilitate the cellular uptake process. The computational results confirmed that the LYCl/DOPE lipid bilayers are stable and also capable of stabilizing DNA fragments via lipoplex formation, with dimensions consistent with experimental values. The optimum formulations (found at 20% of LYCl content) were able to complete the transfection process efficiently and with high cell viabilities, even improving the outcomes of the positive control Lipo2000*. | es_ES |
dc.description.sponsorship | This work was supported by grants from the MINECO of Spain (contract numbers CTQ2015-65972-R, CTQ2015-64425-C2-1-R, CTQ2015-64425-C2-2-R, CTQ2016-80600-P and RTI2018-095844-B-I00), the University Complutense of Madrid (Spain) (project number UCMA05-33-010), and the University of Alcalá (project number CCGP2017-EXP/027). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI AG | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Materias Investigacion::Farmacia::Química farmacéutica | es_ES |
dc.subject | Compaction | es_ES |
dc.subject | Gene delivery | es_ES |
dc.subject | Lipoplex | es_ES |
dc.subject | Lysine-derived cationic lipid | es_ES |
dc.subject | Molecular dynamics | es_ES |
dc.subject | Multilamellar aggregates | es_ES |
dc.subject | Plasmid DNA | es_ES |
dc.subject | Protection | es_ES |
dc.subject | Transfection | es_ES |
dc.title | A Non-Viral Plasmid DNA Delivery System Consisting on a Lysine-Derived Cationic Lipid Mixed with a Fusogenic Lipid | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.description.note | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.identifier.doi | 10.3390/pharmaceutics11120632 | - |
dadun.citation.endingPage | 16 | es_ES |
dadun.citation.number | 632 | es_ES |
dadun.citation.publicationName | Pharmaceutics | es_ES |
dadun.citation.startingPage | 1 | es_ES |
dadun.citation.volume | 11 | es_ES |
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