Purroy, A. (A.)
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- Matrix metalloproteinase-10 effectively reduces infarct size in experimental stroke by enhancing fibrinolysis via a thrombin-activatable fibrinolysis inhibitor-mediated mechanism(Lippincott, Williams & Wilkins, 2011) Parks, W.C. (William C.); Paramo, J.A. (José Antonio); Purroy, A. (A.); Orbe, J. (Josune); Rodriguez, J.A. (José Antonio); Barrenetxe, J. (Jaione); Serrano, R. (Rosario); Martinez-de-Lizarrondo, S. (Sara); Orset, C. (C.); Angles-Cano, E. (Eduardo); Vivien, D. (D.); Birkland, T.P. (T.P.)BACKGROUND: The fibrinolytic and matrix metalloproteinase (MMP) systems cooperate in thrombus dissolution and extracellular matrix proteolysis. The plasminogen/plasmin system activates MMPs, and some MMPs have been involved in the dissolution of fibrin by targeting fibrin(ogen) directly or by collaborating with plasmin. MMP-10 has been implicated in inflammatory/thrombotic processes and vascular integrity, but whether MMP-10 could have a profibrinolytic effect and represent a promising thrombolytic agent is unknown. METHODS AND RESULTS: The effect of MMP-10 on fibrinolysis was studied in vitro and in vivo, in MMP-10-null mice (Mmp10(-/-)), with the use of 2 different murine models of arterial thrombosis: laser-induced carotid injury and ischemic stroke. In vitro, we showed that MMP-10 was capable of enhancing tissue plasminogen activator-induced fibrinolysis via a thrombin-activatable fibrinolysis inhibitor inactivation-mediated mechanism. In vivo, delayed fibrinolysis observed after photochemical carotid injury in Mmp10(-/-) mice was reversed by active recombinant human MMP-10. In a thrombin-induced stroke model, the reperfusion and the infarct size in sham or tissue plasminogen activator-treated animals were severely impaired in Mmp10(-/-) mice. In this model, administration of active MMP-10 to wild-type animals significantly reduced blood reperfusion time and infarct size to the same extent as tissue plasminogen activator and was associated with shorter bleeding time and no intracranial hemorrhage. This effect was not observed in thrombin-activatable fibrinolysis inhibitor-deficient mice, suggesting thrombin-activatable fibrinolysis inhibitor inactivation as one of the mechanisms involved in the MMP-10 profibrinolytic effect. CONCLUSIONS: A novel profibrinolytic role for MMP-10 in experimental ischemic stroke is described, opening new pathways for innovative fibrinolytic strategies in arterial thrombosis.
- Efectos de una intervención dietética sobre el estado nutricional de pacientes en hemodiálisis: diferencias entre varones y mujeres.(Gobierno de Navarra. Departamento de Salud, 1998) Martinez, J.A. (José Alfredo); Sorbet, M.J. (M.J.); Purroy, A. (A.); Solozabal, C. (C.); Marques-Lopes, I. (Iva); Martin, M.M. (María M.)The growing interest in the nutritional status of dialyzed patients arises from the fact that it plays an important role in the evolution, morbidity and mortality of renal patients. In the current study the effects have been assessed of a dietary intervention lasting 6 months, based on a food exchange list, on the nutritional status of a group of 49 hemodialysis patients as well as the different effects between men and women. The initial and final evaluation of the nutritional status was performed by estimating energy and nutrient intake with a 24 hour recall, determination of body composition by anthropometry, bioelectrical impedance and infrared interactance and biochemical measurements related to the nutritional status of the patients undergoing hemodialysis. At the end of the study, a significant increase was seen in the energy and macronutrient intake, which was more prominent in females. The body composition analysis showed a favourable evolution as compared to other studies; which was better in women who maintained the muscle mass stable and increased fat mass. The biochemical profile showed an increase of the C3 complement, while other markers such as amino acid profile and IGF I remained unchanged at the end of the study, being both similar in males and females. The dietary intervention was found to be useful in the prevention of the progression of malnutrition in these patients, apparently with a more beneficial effect on females
- Enfermedad ateroembólica. Presentación de un caso de afectación multisistémica y revisión de la literatura(Universidad de Navarra, 1989) Errasti, P. (Pedro); Diaz-Tejeiro, R. (R.); Bolaños, L. (L.); Purroy, A. (A.); Vaz, O. (O.); Maduell, F. (F.); Esparza, N. (N.)The atheroembolic disease is an entity characterized by obstruction of multiple small arteries by cholesterol crystals. The incidence rises steeply with advancing age and following an invasive procedure involving arteries in patients with atherosclerosis. Actually it has a growing interest due to the increased frequency and to the lack of appropriate therapy. In this paper we report a clinical case of multiple system disease secondary to anticoagulant therapy in a 69 years old patient. We review the pathogenesis, clinical features, laboratory dates and pathology of the disease
- Hepatopatía e insuficiencia renal aguda funcional por rifampicina(Elsevier España, 1978) Otero, R. (R.); Errasti, P. (Pedro); Purroy, A. (A.)Se presenta un caso clínico de hepatopatía funcional y fracaso renal agudo secundario al tratamiento con rifampicina.
- La actividad de la nefrona en la cirrosis hepática(The Spanish Society of Digestive Pathology, 1970) Arroyo, J.L. (José L.); Conchillo, F. (F.); Purroy, A. (A.); Ortiz-de-Landazuri, E. (E.); Bueno, J. (J.)
- Poliquistosis renal y aneurismas cerebrales: Presentación de un caso(Elsevier España, 1978) Otero, R. (R.); Errasti, P. (Pedro); Purroy, A. (A.); Delgado, G. (G.); Cabarcos, A. (A.)Los autores presentan un caso típico de aneurismas arteriales cerebrales asociados a poliquistosis renal de carácter familiar. La rotura de uno de los aneurismas en una sesión de hemodiálisis provocó una hemorragia subaracnoidea que, a pesar del diagnóstico y tratamiento quirúrgico precoz, evolucionó fatalmente.
- Matrix metalloproteinase-10 deficiency delays atherosclerosis progression and plaque calcification(Elsevier, 2018) Belzunce, M. (Miriam); Parks, W.C. (William C.); Paramo, J.A. (José Antonio); Andres, V. (Vicente); Purroy, A. (A.); Orbe, J. (Josune); Rodriguez, J.A. (José Antonio); Villa-Bellosta, R. (Ricardo); Roncal, C. (Carmen); Zalba, G. (Guillermo); Meilhac, O. (Olivier)Background and aims: Matrix metalloproteinases (MMPs) have been implicated in atherosclerosis and vascular calcification. Among them, we reported that MMP10 is present in human atheroma, associated with atherosclerosis. However, it remains unclear whether MMP10 is involved in atherogenesis and vascular calcification. Methods: MMP10 was measured in serum from patients with subclinical atherosclerosis and analyzed in carotid endarterectomies by immunostaining. ApoE-deficient mice (Apoe-/-) were crossed to MMP10-deficient (Mmp10-/-) mice and followed up to 20 months. Plaque area and composition were assessed by histology and immunohistochemistry. Inflammatory markers were measured in atherosclerotic plaques by RT-qPCR, and leukocyte subpopulations were analyzed by flow cytometry. In vitro calcification assays were performed in aortic vascular smooth muscle cells (VSMC). Results: MMP10 serum levels were associated with coronary calcification in subjects with subclinical atherosclerosis. Immunostaining revealed MMP10 expression in human atheromas, spatially associated with calcification areas, and complicated plaques released higher amounts of MMP10 than non-diseased segments. Interestingly, vascular MMP10 expression was confined to the atherosclerotic lesion in Apoe-/- mice, and Apoe-/-Mmp10-/- showed a substantial reduction in atherosclerotic lesion size, macrophage content and plaque calcification. Reduced local and systemic inflammatory markers could be demonstrated in Apoe-/-Mmp10-/- by gene expression and flow cytometry analysis. Calcium phosphate deposition and vascular calcification markers were downregulated in VSMC from Apoe-/-Mmp10-/- mice. Conclusions: Delayed plaque progression and altered cellular composition in the absence of MMP10 suggests that MMP10 plays a role in atherosclerosis, favoring inflammation, development and complication of the plaque.
- Relación anatomoclínica de las glomerulonefritis primitivas(Universidad de Navarra, 1981) Diez-Martinez, J. (Javier); Monfa, J.M. (J. M.); Purroy, A. (A.); Villaro, J. (J.); Sanchez-Ibarrola, A. (Alfonso)
- Estudio radiológico de la osteodistrofia renal(Universidad de Navarra, 1982) Purroy, A. (A.); San-Julian, M. (Mikel); Villaro, J. (J.); Bilbao, J.I. (José I.)
- Inmunidad celular y humoral en pacientes con insuficiencia renal crónica en hemodiálisis periódica(Universidad de Navarra, 1979) Uribarrena, R. (R.); Errasti, P. (Pedro); Purroy, A. (A.); Asin, J.L. (J. L.); Serrano, M. (M.); Cabarcos, A. (A.)