Serrano, J. (Juan)
- Publications
- item.page.relationships.isContributorAdvisorOfPublication
- item.page.relationships.isContributorOfPublication
Search Results
Now showing 1 - 1 of 1
- S-Nitrosation of proteins during D-galactosamine-induced cell death in human hepatocytes(Informa Healthcare, 2007) Rodriguez-Ariza, A. (Antonio); Corrales, F.J. (Fernando José); Muntane, J. (Jordi); Fraga, E. (Enrique); Lopez-Sanchez, L.M. (Laura M.); Montero, J.L. (José L.); Collado, J.A. (Juan A.); Serrano, J. (Juan); Lopez-Cillero, P. (Pedro); Mata, M. (Manuel) de laNitric oxide (NO) participates in the cell death induced by d-Galactosamine (d-GalN) in hepatocytes, and NO-derived reactive oxygen intermediates are critical contributors to protein modification and hepatocellular injury. It is anticipated that S-nitrosation of proteins will participate in the mechanisms leading to cell death in d-GalN-treated human hepatocytes. In the present study, d-GalN-induced cell death was related to augmented levels of NO production and S-nitrosothiol (SNO) content. The biotin switch assay confirmed that d-GalN increased the levels of S-nitrosated proteins in human hepatocytes. S-nitrosocysteine (CSNO) enhanced protein S-nitrosation and altered cell death parameters that were related to S-nitrosation of the executioner caspase-3. Fifteen S-nitrosated proteins participating in metabolism, antioxidative defense and cellular homeostasis were identified in human hepatocytes treated with CSNO. Among them, seven were also identified in d-GalN-treated hepatocytes. The results here reported underline the importance of the alteration of SNO homeostasis during d-GalN-induced cell death in human hepatocytes