Pozo, M.A. (Miguel Ángel)

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    Construction of different radionuclide templates of rat brains and their use on a new statistic parametric mapping analysis protocol for PET studies. | International WorkshopCNA´10: Bio-medical applications of Micro-PET (20-21 September 2010. Sevilla, Spain)
    (2010-09-20) Pozo, M.A. (Miguel Ángel); Delgado, M. (Mercedes); Juri, C. (Carlos); Collantes, M. (María); Peñuelas-Sanchez, I. (Ivan); Prieto-Azcárate, E. (Elena); Molinet-Dronda, F. (Francisco); Fernandez, M.E. (M. E.); Garcia-Garcia, L. (Luis)
    This work shows the development of protocols to create new 18F‐FDG and 11C‐DTBZ (dyhidrotetrabenazine, a VMAT2 transporter ligand) templates of rat brain for spatial normalisation and definition of standardised areas in images used for setting up SPM analysis of PET data.
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    Statistical parametric maps of (18)F-FDG PET and 3-D autoradiography in the rat brain: a cross-validation study
    (Springer Verlag, 2011) Pozo, M.A. (Miguel Ángel); Delgado, M. (Mercedes); Marti-Climent, J.M. (Josep María); Juri, C. (Carlos); Obeso, J.A. (José A.); Collantes, M. (María); Peñuelas-Sanchez, I. (Ivan); Prieto-Azcárate, E. (Elena); Molinet-Dronda, F. (Francisco); Gago, B. (Belén); Fernandez-Valle, M.E. (M. E.); Garcia-Garcia, L. (Luis)
    PURPOSE: Although specific positron emission tomography (PET) scanners have been developed for small animals, spatial resolution remains one of the most critical technical limitations, particularly in the evaluation of the rodent brain. The purpose of the present study was to examine the reliability of voxel-based statistical analysis (Statistical Parametric Mapping, SPM) applied to (18)F-fluorodeoxyglucose (FDG) PET images of the rat brain, acquired on a small animal PET not specifically designed for rodents. The gold standard for the validation of the PET results was the autoradiography of the same animals acquired under the same physiological conditions, reconstructed as a 3-D volume and analysed using SPM. METHODS: Eleven rats were studied under two different conditions: conscious or under inhalatory anaesthesia during (18)F-FDG uptake. All animals were studied in vivo under both conditions in a dedicated small animal Philips MOSAIC PET scanner and magnetic resonance images were obtained for subsequent spatial processing. Then, rats were randomly assigned to a conscious or anaesthetized group for postmortem autoradiography, and slices from each animal were aligned and stacked to create a 3-D autoradiographic volume. Finally, differences in (18)F-FDG uptake between conscious and anaesthetized states were assessed from PET and autoradiography data by SPM analysis and results were compared. RESULTS: SPM results of PET and 3-D autoradiography are in good agreement and led to the detection of consistent cortical differences between the conscious and anaesthetized groups, particularly in the bilateral somatosensory cortices. However, SPM analysis of 3-D autoradiography also highlighted differences in the thalamus that were not detected with PET. CONCLUSION: This study demonstrates that any difference detected with SPM analysis of MOSAIC PET images of rat brain is detected also by the gold standard autoradiographic technique, confirming that this methodology provides reliable results, although partial volume effects might make it difficult to detect slight differences in small regions.
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    Decreased glucose-derivate uptake in primary somatosensorial cortex in the brain of female AIP mice. | International Porphyrins and Porphyria Meeting (10-13 April 2011. Cardiff, Wales, UK)
    (2011-04-13) Pozo, M.A. (Miguel Ángel); Delgado, M. (Mercedes); Benito, M. (Marina); Collantes, M. (María); Lanciego, J.L. (José Luis); Peñuelas-Sanchez, I. (Ivan); Desco, M. (Manuel); Prieto-Azcárate, E. (Elena); Molinet-Dronda, F. (Francisco); Unzu, C. (Carmen); Prieto, J. (Jesús); Enriquez-de-Salamanca, R. (Rafael); Fontanellas-Romá, A. (Antonio); Garcia-Garcia, L. (Luis); Rodriguez, I. (Ignacio)