Fernandez-Lago, L. (Luis)

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1982 - 198922020 - 20231

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    Demonstration of antibodies against Brucella melitensis 16M lipopolysaccharide and native hapten in human sera by enzyme-linked immunosorbent assay
    (American Society for Microbiology, 1986) Diaz, R. (Ramón); Fernandez-Lago, L. (Luis)
    An enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of human immunoglobulin G (IgG), IgA, and IgM antibodies against Brucella melitensis 16M by using lipopolysaccharide (LPS) and native hapten (NH) as antigens is described. The results obtained with the LPS ELISA were compared with the results of the NH ELISA. A good statistically significant correlation was established between the antibody titers of the IgG class against both antigens. A total of 104 (99%) of the 105 serum samples of patients with brucellosis exhibited specific anti-NH antibodies by the ELISA technique. In 52 (50%) of these positive samples, antibodies against NH were detected by radial immunodiffusion (RID). In 100% of these RID-positive sera, the antibody titers of the IgG class with ELISA-determined anti-NH specificity were equal to or greater than 160. These results point to a higher sensitivity of the ELISA technique as compared with RID. Inhibition experiments revealed that the assay was specific for LPS and NH from B. melitensis 16M.
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    If You're Not Confused, You're Not Paying Attention: Ochrobactrum Is Not Brucella
    (2023) Bertu, W.J. (Wilson J.); Güler, L. (Leyla); Caswell, C.C. (Clayton C.); Araj, G.F. (George F.); Suárez-Esquivel, M. (Marcela); Lopez-Goñi, I. (Ignacio); Al-Dahouk, S. (Sascha); Roop, M. (Martin); Pembroke, J.T. (J. Tony); Chacon-Diaz, C. (Carlos); Middlebrook, E.A. (Edgar A.); Blasco, J.M. (José María); Loperena-Barber, M. (Maite); Keriel, A. (Anne); Salvador-Bescós, M. (Miriam); Dadar, M. (Maryam); O'Callaghan, D. (David); Moriyon, I. (Ignacio); De-Massis, F. (Frabizio); Altamirano-Silva, P. (Pamela); Barquero-Calvo, E. (Elías); Chaves-Olarte, E. (Esteban); Neubauer, H. (Heinrich); Whatmore, A.M. (Adrian M.); Wareth, G. (Gamal); De-Lima-Santos, R. (Renato); Arenas-Gamboa, A. (Ángela); Welburn, S.C. (Susan C.); Godfroid, J. (Jacques); Diaz, R. (Ramón); Splitter, G. (Gary); Garin-Bastuji, B. (B.); Gusi, A.M. (Amahyel M.); Sangari, F.J. (Félix Javier); Melzer, F. (Falk); Comerci, D.J. (Diego J.); Salcedo, S.P. (Suzana P.); Arce-Gorvel, V. (Vilma); Zuñiga-Ripa, A. (Amaia); Vizcaíno, N. (Nieves); Ruiz-Villalonos, N. (Nazaret); Erdenlig-Gürbilek, S. (Sevil); Muñoz, P. (Pilar); Tsolis, R.M. (Renee M.); Mora-Cartin, R. (Ricardo); Gorvel, J.P. (Jean Pierre); Ryan, M.P. (Michael P.); Iriarte-Cilveti, M. (Maite); Seimenis, A. (Aristarchos); Tabbaa, D. (Darem); Khames, M. (Mammar); Cravero, S. (Silvio); Celli, J. (Jean); Moran-Gilad, J. (Jacob); Bosilkovski, M. (Mile); Letesson, J.J. (Jean Jacques); Cook, E. (Elizabeth); Oñate-Landa, A.(A.); Moreno, E. (Edgardo); Ariza, J. (J.); Pandey, P. (Piyush); Escobar, G.I. (Gabriela I.); McGiven, J. (John); Guzman-Verri, C. (Caterina); Trangoni, M.D. (Marcos David); Pappas, G. (Georgios); Köhler, S. (Stephan); Foster, J.T. (Jeffrey T.); De-Boelle, X. (Xavier); Hernández-Mora, G. (Gabriela); Conde-Alvarez, R. (Raquel); Cadmus, S. (Simeon); Battelli, G. (Giorgio); Ficht, T.A. (Thomas A.); Hai, J. (Jiang); Jacob, N.R. (Nestor R.); Ocholi, R.A. (Reuben A.); Fernandez-Lago, L. (Luis)
    Bacteria of the genus Brucella are facultative intracellular parasites that cause brucellosis, a severe animal and human disease. Recently, a group of taxonomists merged the brucellae with the primarily free-living, phylogenetically related Ochrobactrum spp. in the genus Brucella. This change, founded only on global genomic analysis and the fortuitous isolation of some opportunistic Ochrobactrum spp. from medically compromised patients, has been automatically included in culture collections and databases. We argue that clinical and environmental microbiologists should not accept this nomenclature, and we advise against its use because (i) it was presented without in-depth phylogenetic analyses and did not consider alternative taxonomic solutions; (ii) it was launched without the input of experts in brucellosis or Ochrobactrum; (iii) it applies a non-consensus genus concept that disregards taxonomically relevant differences in structure, physiology, population structure, core-pangenome assemblies, genome structure, genomic traits, clinical features, treatment, prevention, diagnosis, genus description rules, and, above all, pathogenicity; and (iv) placing these two bacterial groups in the same genus creates risks for veterinarians, medical doctors, clinical laboratories, health authorities, and legislators who deal with brucellosis, a disease that is particularly relevant in low- and middle-income countries. Based on all this information, we urge microbiologists, bacterial collections, genomic databases, journals, and public health boards to keep the Brucella and Ochrobactrum genera separate to avoid further bewilderment and harm.
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    Immunological identity of brucella native hapten, polysaccharide B, and yersinia enterocolitica serotype 9 native hapten
    (American Society for Microbiology, 1982) Moriyon, I. (Ignacio); Toyos, J. (J.); Fernandez-Lago, L. (Luis)
    Yersinia enterocolitica serotype 9 contained an antigenic component giving a reaction of total identity with Brucella native hapten and polysaccharide B. This component was present in a phenol-water extract (fraction 5; M. Redfearn, Ph.D. Thesis, University of Wisconsin, Madison, 1960) along with the smooth lipopolysaccharide. The native hapten could be purified free of lipopolysaccharide and proteins by gel filtration.