Ribeiro, R. (Ricardo)

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    Obesity and prostate cancer: gene expression signature of human periprostatic adipose tissue
    (BioMed Central, 2012) Principe, P. (Paulo); Pina, F. (Francisco); Monteiro, C. (Cátia); Medeiros, R. (Rui); Sanches-Magalhães, J. (José); Catalan, V. (Victoria); Fraga, A. (Avelino); Morais, A. (Antonio); Oliveira, J. (Jorge); Ribeiro, R. (Ricardo); Frühbeck, G. (Gema); Lobato, C. (Carlos); Gomez-Ambrosi, J. (Javier); Lobo, F. (Francisco); Rodriguez, A. (Amaia); Cunha, V. (Virginia); Lopes, C. (Carlos); Hu, P. (Pingzhao); Silva, V. (Vitor)
    Obesity and excess adiposity modified the expression of PP adipose tissue genes to ultimately foster fat mass growth. In patients with prostate cancer the expression profile of PP adipose tissue accounted for hypercellularity and reduced immunosurveillance. Both findings may be liable to promote a favorable environment for prostate cancer progression.
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    Epigenome-wide DNA methylation profiling of periprostatic adipose tissue in prostate cancer patients with excess adiposity-a pilot study
    (BioMed Central, 2018) Matos, A. (Andreia); Monteiro, C. (Cátia); Cheng, Y. (Yan); Catalan, V. (Victoria); Fraga, A. (Avelino); Ribeiro, R. (Ricardo); Frühbeck, G. (Gema); Pereira, C. (Carina); You, J. (Jiaying); Gomez-Ambrosi, J. (Javier); Rodriguez, A. (Amaia); Hu, P. (Pingzhao)
    Background: Periprostatic adipose tissue (PPAT) has been recognized to associate with prostate cancer (PCa) aggressiveness and progression. Here, we sought to investigate whether excess adiposity modulates the methylome of PPAT in PCa patients. DNA methylation profiling was performed in PPAT from obese/overweight (OB/OW, BMI > 25 kg m−2 ) and normal weight (NW, BMI < 25 kg m−2 ) PCa patients. Significant differences in methylated CpGs between OB/OW and NW groups were inferred by statistical modeling. Results: Five thousand five hundred twenty-six differentially methylated CpGs were identified between OB/OW and NW PCa patients with 90.2% hypermethylated. Four hundred eighty-three of these CpGs were found to be located at both promoters and CpG islands, whereas the representing 412 genes were found to be involved in pluripotency of stem cells, fatty acid metabolism, and many other biological processes; 14 of these genes, particularly FADS1, MOGAT1, and PCYT2, with promoter hypermethylation presented with significantly decreased gene expression in matched samples. Additionally, 38 genes were correlated with antigen processing and presentation of endogenous antigen via MHC class I, which might result in fatty acid accumulation in PPAT and tumor immune evasion. Conclusions: Results showed that the whole epigenome methylation profiles of PPAT were significantly different in OB/OW compared to normal weight PCa patients. The epigenetic variation associated with excess adiposity likely resulted in altered lipid metabolism and immune dysregulation, contributing towards unfavorable PCa microenvironment, thus warranting further validation studies in larger samples.