Andres, D. (D.) de

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    The extradomain A of fibronectin (EDA) combined with poly(I:C) enhances the immune response to HIV-1 p24 protein and the protection against recombinant Listeria monocytogenes-Gag infection in the mouse model
    (Elsevier, 2012) Asensio, A.C. (Aaron C.); San-Roman, B. (Beatriz); Garrido, V. (Victoria); Mansilla, C. (Cristina); Andres, D. (D.) de; Muñoz, P. (Pilar); Amorena, B. (Beatriz); Obregon, P. (Patricia); Andres, X. (Ximena) de; Arribillaga, L. (Laura); Grillo, M.J. (María Jesús); Lasarte, J.J. (Juan José)
    The development of effective vaccines against HIV-1 infection constitutes one of the major challenges in viral immunology. One of the protein candidates in vaccination against this virus is p24, since it is a conserved HIV antigen that has cytotoxic and helper T cell epitopes as well as B cell epitopes that may jointly confer enhanced protection against infection when used in immunization-challenge approaches. In this context, the adjuvant effect of EDA (used as EDAp24 fusion protein) and poly(I:C), as agonists of TLR4 and TLR3, respectively, was assessed in p24 immunizations using a recombinant Listeria monocytogenes HIV-1 Gag proteins (Lm-Gag, where p24 is the major antigen) for challenge in mice. Immunization with EDAp24 fusion protein together with poly(I:C) adjuvant induced a specific p24 IFN-γ production (Th1 profile) as well as protection against a Lm-Gag challenge, suggesting an additive or synergistic effect between both adjuvants. The combination of EDA (as a fusion protein with the antigen, which may favor antigen targeting to dendritic cells through TLR4) and poly(I:C) could thus be a good adjuvant candidate to enhance the immune response against HIV-1 proteins and its use may open new ways in vaccine investigations on this virus.
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    Protection from Staphylococcus aureus mastitis associated with poly-N-acetyl beta-1,6 glucosamine specific antibody production using biofilm-embedded bacteria
    (Elsevier, 2009) Lasa, I. (Íñigo); Maira-Litran, T. (T.); Solano, C. (C.); Penades, J. (J.); Marco, J. (J.); Andres, D. (D.) de; Amorena, B. (Beatriz); Prenafeta, A. (A.); Costa, L. (L.); Pier, G.B. (G.B.); Jimenez-Barbero, J. (J.); Irache, J.M. (Juan Manuel); Perez-Calvo, M.M. (M.M.); Rota, C. (C.); Grillo, M.J. (María Jesús); Valle, J. (Jaione)
    Staphylococcus aureus vaccines based on bacterins surrounded by slime, surface polysaccharides coupled to protein carriers and polysaccharides embedded in liposomes administered together with non-biofilm bacterins confer protection against mastitis. However, it remains unknown whether protective antibodies are directed to slime-associated known exopolysaccharides and could be produced in the absence of bacterin immunizations. Here, a sheep mastitis vaccination study was carried out using bacterins, crude bacterial extracts or a purified exopolysaccharide from biofilm