Correa-de-Sa, M.I. (M. I.)
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- Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains(American Society for Microbiology, 2008) Lopez-Goñi, I. (Ignacio); Cloeckaert, A. (Axel); Miguel, M.J. (María Jesús) de; Garin-Bastuji, B. (Bruno); Correa-de-Sa, M.I. (M. I.); Marin, C.M. (C. M.); Muñoz, P. (Pilar); Garcia-Yoldi, D. (David); Jacques, I. (I.); Cardoso, R. (R.); Blasco, J.M. (J. M.); Walravens, K. (K.); Albert, D. (D.); Grayon, M. (M.); Ferreira, A.C. (A. C.)An evaluation of a multiplex PCR assay (Bruce-ladder) was performed in seven laboratories using 625 Brucella strains from different animal and geographical origins. This robust test can differentiate in a single step all of the classical Brucella species, including those found in marine mammals and the S19, RB51, and Rev.1 vaccine strains.