Laharrague, P. (Patrick)

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    Transplantation of adipose derived stromal cells is associated with functional improvement in a rat model of chronic myocardial infarction
    (Oxford University Press, 2008-05) Joffre, C. (Carine); Cemborain, A. (A.); Collantes, M. (María); Peñuelas-Sanchez, I. (Ivan); Gavira, J.J. (Juan José); Planat-Benard, V. (Valérie); Boisson, M. (M.); Laharrague, P. (Patrick); Leobon, B. (Bertrand); Casteilla, L. (L.); Barba, J. (Joaquín); Ecay, M. (Margarita); Mazo, M. (Manuel); Prosper-Cardoso, F. (Felipe); Pelacho, B. (Beatriz); Penicaud, L. (Luc); Abizanda-Sarasa, G. (Gloria)
    Aims: To determine the effect of transplantation of undifferentiated and cardiac pre-differentiated adipose stem cells compared with bone marrow mononuclear cells (BM-MNC) in a chronic model of myocardial infarction. Methods: Ninety-five Sprague–Dawley rats underwent left coronary artery ligation and after 1month received by direct intramyocardial injection either adipose derived stem cells (ADSC), cardiomyogenic cells (AD-CMG) or BM-MNC from enhanced-Green Fluorescent Protein (eGFP) mice. The control group was treated with culture medium. Heart function was assessed by echocardiography and 18F-FDG microPET. Cell engraftment, differentiation, angiogenesis and fibrosis in the scar tissue were also evaluated by (immuno)histochemistry and immunofluorescence. Results: One month after cell transplantation, ADSC induced a significant improvement in heart function (LVEF 46.3±9.6% versus 27.7±8% pre-transplant) and tissue viability (64.78±7.2% versus 55.89±6.3% pre-transplant). An increase in the degree of angiogenesis and a decrease in fibrosis were also detected. Although transplantation of AD-CMG or BM-MNC also had a positive, albeit smaller, effect on angiogenesis and fibrosis in the infarcted hearts, this benefit did not translate into a significant improvement in heart function or tissue viability. Conclusion: These results indicate that transplantation of adipose derived cells in chronic infarct provides a superior benefit to cardiac pre-differentiated ADSC and BM-MNC.