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dc.creatorNguewa, P.A. (Paul Alain)-
dc.creatorAgorreta, J. (Jackeline)-
dc.creatorBlanco, D. (D.)-
dc.creatorLozano, M.D. (María Dolores)-
dc.creatorGomez-Roman, J. (Javier)-
dc.creatorSanchez, B.A. (Blas A.)-
dc.creatorValles, I. (Iñaki)-
dc.creatorPajares, M.J. (María José)-
dc.creatorPio, R. (Rubén)-
dc.creatorRodriguez, M.J. (María José)-
dc.creatorMontuenga-Badia, L.M. (Luis M.)-
dc.creatorCalvo-González, A. (Alfonso)-
dc.date.accessioned2010-10-21T09:55:57Z-
dc.date.available2010-10-21T09:55:57Z-
dc.date.issued2008-11-17-
dc.identifier.citationNguewa PA, Agorreta J, Blanco D, Lozano MD, Gomez-Roman J, Sanchez BA, et al. Identification of importin 8 (IPO8) as the most accurate reference gene for the clinicopathological analysis of lung specimens. BMC Mol Biol 2008 Nov 17;9:103.es_ES
dc.identifier.issn1471-2199-
dc.identifier.urihttps://hdl.handle.net/10171/13592-
dc.description.abstractAbstract Background: The accurate normalization of differentially expressed genes in lung cancer is essential for the identification of novel therapeutic targets and biomarkers by real time RT-PCR and microarrays. Although classical "housekeeping" genes, such as GAPDH, HPRT1, and beta-actin have been widely used in the past, their accuracy as reference genes for lung tissues has not been proven. Results: We have conducted a thorough analysis of a panel of 16 candidate reference genes for lung specimens and lung cell lines. Gene expression was measured by quantitative real time RTPCR and expression stability was analyzed with the softwares GeNorm and NormFinder, mean of |ΔCt| (= |Ct Normal-Ct tumor|) ± SEM, and correlation coefficients among genes. Systematic comparison between candidates led us to the identification of a subset of suitable reference genes for clinical samples: IPO8, ACTB, POLR2A, 18S, and PPIA. Further analysis showed that IPO8 had a very low mean of |ΔCt| (0.70 ± 0.09), with no statistically significant differences between normal and malignant samples and with excellent expression stability. Conclusion: Our data show that IPO8 is the most accurate reference gene for clinical lung specimens. In addition, we demonstrate that the commonly used genes GAPDH and HPRT1 are inappropriate to normalize data derived from lung biopsies, although they are suitable as reference genes for lung cell lines. We thus propose IPO8 as a novel reference gene for lung cancer samples.es_ES
dc.language.isoenges_ES
dc.publisherBioMed Centrales_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectIdentificationes_ES
dc.subjectLung specimenses_ES
dc.subjectIPO-8es_ES
dc.subjectThe most accurate reference genees_ES
dc.subjectClinicopathological analysises_ES
dc.titleIdentification of importin (IPO-8) as the most accurate reference gene for the clinicopathological analysis of lung specimenses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doihttp://dx.doi.org/10.1186/1471-2199-9-103es_ES

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