Full metadata record
DC Field | Value | Language |
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dc.creator | Kawamata, N. (N.) | |
dc.creator | Ogawa, S. (Seishi) | |
dc.creator | Gueller, S. (S.) | |
dc.creator | Ross, S.H. (Samuel H.) | |
dc.creator | Huynh, T. (T.) | |
dc.creator | Chen, J. (J.) | |
dc.creator | Chang, A. (A.) | |
dc.creator | Nabavi-Nouis, S. (Shayan) | |
dc.creator | Megrabian, N. (Nairi) | |
dc.creator | Siebert, R. (Reiner) | |
dc.creator | Martinez-Climent, J.A. (José Ángel) | |
dc.creator | Koeffler, H.P. (H. Philip) | |
dc.date.accessioned | 2011-05-06T16:36:21Z | - |
dc.date.available | 2011-05-06T16:36:21Z | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Kawamata N, Ogawa S, Gueller S, Ross SH, Huynh T, Chen J, et al. Identified hidden genomic changes in mantle cell lymphoma using high-resolution single nucleotide polymorphism genomic array. Exp Hematol 2009 Aug;37(8):937-946. | es_ES |
dc.identifier.issn | 1873-2399 | - |
dc.identifier.uri | https://hdl.handle.net/10171/17893 | - |
dc.description.abstract | OBJECTIVE: Mantle cell lymphoma (MCL) is a lymphoma characterized by aberrant activation of CCND1/cyclin D1 followed by sequential genetic abnormalities. Genomic abnormalities in MCL have been extensively examined by classical cytogenetics and microarray-based comparative genomic hybridization techniques, pointing out a number of alterations in genomic regions that correlate with the neoplastic phenotype and survival. Recently, single nucleotide polymorphism genomic microarrays (SNP-chip) have been developed and used for analysis of cancer genomics. This technique allows detection of genomic changes with higher resolution, including loss of heterozygosity without changes of gene dosage, so-called acquired uniparental disomy (aUPD). MATERIALS AND METHODS: We have examined 33 samples of MCL (28 primary MCL and 5 cell lines) using the 250,000 SNP-chip from Affymetrix. RESULTS: Known alterations were confirmed by SNP arrays, including deletion of INK4A/ARF, duplication/amplification of MYC, deletion of ATM, and deletion of TP53. We also identified a duplication/amplification that occurred at 13q involving oncogenic microRNA, miR17-92. We found other genomic abnormalities, including duplication/amplification of cyclin D1, del(1p), del(6q), dup(3q) and dup(18q). Our SNP-chip analysis detected these abnormalities at high resolution, allowing us to narrow the size of the commonly deleted regions, including 1p and 6q. Our SNP-chip analysis detected a number of aUPD sites, including whole chromosome 9 aUPD and 9p aUPD. We also found an MCL case with 19p, leading to homozygous deletion of TNFSF genes. CONCLUSION: SNP-chip analysis detected in MCL very small genomic gains/losses, as well as aUPDs, which could not be detected by more conventional methods. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/closedAccess | - |
dc.subject | DNA mutational analysis | es_ES |
dc.subject | Gene deletion | es_ES |
dc.subject | Gene duplication | es_ES |
dc.subject | Lymphoma, Mantle-cell | es_ES |
dc.title | Identified hidden genomic changes in mantle cell lymphoma using high-resolution single nucleotide polymorphism genomic array | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
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