Isolation, culture and characterization of adult carotid body-derived cells
Keywords: 
Carotid body cells
Cell theraphy
Parkinson disease
Issue Date: 
2009
Publisher: 
Elsevier
ISSN: 
1569-9048
Citation: 
Izal-Azcarate, A., Gutierrez-Perez, M., Izal, I., Belzunegui, S. et al.Isolation, culture and characterization of adult carotid body-derived cells. Respiratory Physiology & Neurobiology (2009); 167: 201–207
Abstract
Recent studies indicate that carotid body (CB) could be a suitable cell source for cell therapy in Parkinson’s disease.We have isolated and successfully expanded in culture as monolayer adult CB-derived cells using a modification of the culture medium employed for bone marrow multipotent adult progenitor cells (MAPCs). These cells express variable amounts of tyrosine hydroxylase (TH), -III tubulin and Sox2. In addition, CB-derived cells showed high expression of Sox2 related to a high rate of proliferation and consistent with an undifferentiated state. Under culture conditions that reduced cell proliferation, Sox2 expression decreased while TH and -III tubulin expression was increased. This could indicate that the differentiation of some cells occurs in the culture, thus accounting for a certain neural differentiation potential of CB-derived cells.

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