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dc.creatorRice, C.M. (Claire .M)-
dc.creatorClavel, C. (C.)-
dc.creatorMazo, M. (Manuel)-
dc.creatorProsper, F. (Felipe)-
dc.creatorScolding, N.J. (Neil J.)-
dc.date.accessioned2011-05-25T15:06:30Z-
dc.date.available2011-05-25T15:06:30Z-
dc.date.issued2010-
dc.identifier.citationRice, C. M., Clavel, C., Mazo, M., Prosper, F. et al.Multipotent adult progenitor cell isolation and proliferation in cytokine and serum-free medium conditioned by rat B104 cells. Br. J. Haematol. 2010; 148 (3): 441–444es_ES
dc.identifier.issn0007-1048-
dc.identifier.urihttps://hdl.handle.net/10171/18156-
dc.description.abstractThis report describes the isolation of rodent multipotent adult progenitor cells (MAPCs) and proliferation of these cells in both standard medium and medium without exogenous serum or growth factors conditioned by the rat cell line B104. MAPCs have exacting requirements for their proliferation in vitro but once established proliferate rapidly at low seeding density, requiring almost daily passage and media exchange. Previously published methods for growth of MAPCs in vitro all used media supplemented with serum and growth factors, which adds considerable expense.es_ES
dc.language.isoeng-
dc.publisherWiley-Blackwelles_ES
dc.rightsinfo:eu-repo/semantics/closedAccess-
dc.subjectMultipotent adult progenitor cells,es_ES
dc.subjectB104es_ES
dc.subjectCell culturees_ES
dc.subjectCell growthes_ES
dc.subjectProliferationes_ES
dc.subjectMaterias Investigacion::Ciencias de la Salud::Oncologíaes_ES
dc.titleMultipotent adult progenitor cell isolation and proliferation in cytokine and serum-free medium conditioned by rat B104 cellses_ES
dc.typeArticuloes_ES
dc.identifier.doihttp://dx.doi.org/10.1111/j.1365-2141.2009.07986.xes_ES

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