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dc.creatorMartinez-Forero, I. (Iván)-
dc.creatorGarcia-Muñoz, R. (R.)-
dc.creatorMartinez-Pasamar, S. (Sara)-
dc.creatorInoges, S. (Susana)-
dc.creatorLopez-Diaz-de-Cerio, A. (Ascensión)-
dc.creatorPalacios, R. (Ricardo)-
dc.creatorSepulcre, J. (Jorge)-
dc.creatorMoreno, B. (Beatriz)-
dc.creatorGonzalez, Z. (Zaira)-
dc.creatorFernandez-Diez, B. (B.)-
dc.creatorMelero, I. (Ignacio)-
dc.creatorBendandi, M. (Maurizio)-
dc.creatorVilloslada, P. (Pablo)-
dc.identifier.citationMartínez-Forero I, García-Muñoz R, Martínez-Pasamar S, Inoges S, López-Díaz de Cerio A, Palacios R, et al. IL-10 suppressor activity and ex vivo Tr1 cell function are impaired in multiple sclerosis. Eur J Immunol 2008 Feb;38(2):576-586.es_ES
dc.description.abstractT regulatory cells type 1 (Tr1 cells) are excellent candidates for cell therapy in multiple sclerosis (MS). The aim of our study was to assess the functional state of Tr1 cells and IL-10R signaling in patients with MS. Tr1 cells were induced in vitro by activation with anti-CD46 antibodies in controls and patients with MS. Cells were phenotyped by cytometry and suppression assays, and the expression of cytokines and transcription factors was evaluated by real-time PCR, ELISA, cytometry and Western blotting. We found that the activity of Tr1 cells and IL-10R signaling is impaired in MS patients since Tr1 cells isolated from MS patients produced less IL-10 than those obtained from controls. Indeed, the supernatants from Tr1 cells from controls did not suppress the proliferation of stimulated CD4(+) cells from patients with MS. Furthermore, the IL-10R signaling pathway was not fully active in CD4(+) cells from MS patients and these cells had higher baseline levels of SOCS3 transcripts than controls. Indeed, after in vitro IL-10 stimulation, the expression levels of the STAT1, STAT3 and IL-10RA genes were higher in MS patients than in controls. Moreover, Stat-3 phosphorylation was lower in controls than in patients after IL-10 stimulation. These results indicate that IL-10 regulatory function is impaired in patients with MS.es_ES
dc.subjectTr1 cells Abbreviations: c(t): cycle threshold HC: healthy control MNE: mean normalized expression MS: multiple sclerosis Socs: suppressor of cytokine signaling Tr1 cell: T regulatory cell type 1 576 Ivan Martinez-Forero et al. Eur. J. Immunol. 2008. 38: 576–586 f 2008es_ES
dc.titleIL-10 suppressor activity and ex vivo Tr1 cell function are impaired in multiple sclerosises_ES

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