Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Lopez-Diaz-de-Cerio, A. (Ascensión) | - |
dc.creator | Lasarte, J.J. (Juan José) | - |
dc.creator | Casares, N. (Noelia) | - |
dc.creator | Sarobe, P. (Pablo) | - |
dc.creator | Ruiz, M. (Marta) | - |
dc.creator | Prieto, J. (Jesús) | - |
dc.creator | Borras-Cuesta, F. (Francisco) | - |
dc.date.accessioned | 2012-01-16T09:45:10Z | - |
dc.date.available | 2012-01-16T09:45:10Z | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | Lopez-Diaz de Cerio A, Lasarte JJ, Casares N, Sarobe P, Ruiz M, Prieto J, et al. Engineering Th determinants for efficient priming of humoral and cytotoxic T cell responses. Int Immunol 2003 Jun;15(6):691-699. | es_ES |
dc.identifier.issn | 0953-8178 | - |
dc.identifier.uri | https://hdl.handle.net/10171/20437 | - |
dc.description.abstract | To engineer T(h) determinants (THd) to prime help for humoral or cytotoxic T cell responses, we modified ovalbumin [OVA(323-337)] and myoglobin [MYO(106-118)] eliciting T(h)1 and T(h)0 cytokine profiles respectively. Residues along the sequence of both THd were replaced with amino acids representative of different families. Replacements at positions P-1 and P5 pointing to the TCR in both THd afforded higher levels of IFN-gamma and IL-4 production. Peptides eliciting different proportions of IFN-gamma and IL-4 were co-immunized with a peptide hapten or a T cytotoxic determinant (TCd) respectively. OVA(323-337)- and MYO(106-118)-derived peptides afforded the best THd for the induction of cytotoxic T lymphocyte (CTL) and anti-hapten antibodies respectively. IFN-gamma and IL-4, primed by MYO(106-118)-derived peptides, correlated significantly with antibody production against the hapten (P < 0.05 for IFN-gamma and P < 0.05 for IL-4). Interestingly, two peptides derived from OVA(323-337), 323G and 327G, which induced the clearest T(h)2 cytokine profiles, were not the most efficient to prime cell help for the induction of anti-hapten antibodies. For CTL induction, OVA(323-337)-derived peptides, inducing a T(h)1-like profile, required a lower dose (5 nmol) than T(h)0 peptides (50 nmol). The dose of 50 nmol was detrimental for T(h)1-like peptides. Interestingly, IFN-gamma primed by the THd correlated significantly with that induced by the TCd (P < 0.01). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Oxford University Press | es_ES |
dc.rights | info:eu-repo/semantics/closedAccess | - |
dc.subject | Antibody | es_ES |
dc.subject | Antigen | es_ES |
dc.subject | Cytotoxicity | es_ES |
dc.subject | Epitope | es_ES |
dc.subject | Peptide | es_ES |
dc.subject | Th1 | es_ES |
dc.subject | Th2 | es_ES |
dc.subject | TCR | es_ES |
dc.title | Engineering Th determinants for efficient priming of humoral and cytotoxic T cell responses | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | http://dx.doi.org/10.1093/intimm/dxg071 | es_ES |
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