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dc.creatorPrieto, I. (Isidro)-
dc.creatorHervas-Stubbs, S. (Sandra)-
dc.creatorGarcia-Granero, M. (Marta)-
dc.creatorBerasain, C. (Carmen)-
dc.creatorRiezu-Boj, J.I. (José Ignacio)-
dc.creatorLasarte, J.J. (Juan José)-
dc.creatorSarobe, P. (Pablo)-
dc.creatorPrieto, J. (Jesús)-
dc.creatorBorras-Cuesta, F. (Francisco)-
dc.identifier.citationPrieto I, Hervas-Stubbs S, Garcia-Granero M, Berasain C, Riezu-Boj JI, Lasarte JJ, et al. Simple strategy to induce antibodies of distinct specificity: application to the mapping of gp120 and inhibition of HIV-1 infectivity. Eur J Immunol 1995 Apr;25(4):877-883.es_ES
dc.description.abstractIn this study 96 15-mer peptides encompassing the entire sequence of HIV-1 gp120 were synthesized and used to immunize BALB/c mice (i) alone or (ii) in conjunction with the T helper cell determinant FISEAIIHVLHSR (FIS) from sperm whale myoglobin, which is well recognized by major histocompatibility complex (MHC) class II molecules of BALB/c. Of these peptides 39 were immunogenic per se and 57 were not. Out of the 57 non-immunogenic peptides 53 could be rendered immunogenic with the second immunization protocol. With the exception of 4 cases, the anti-peptide antibody titers induced in (ii) were equal (14 cases) or higher (78 cases) than those induced in (i). From the 96 anti-peptide antibodies tested, 12 were able to recognize recombinant gp120 with good antibody titers, a result in agreement with previously identified B cell epitopes from gp120 by anti-peptide antibodies induced with longer peptides conjugated to a carrier protein. Moreover, 4 of the 12 anti-peptide antisera that recognized gp 120 were able to neutralize HIV-1 infectivity in vitro, showing that the strategy of co-immunization with FIS may afford functional antibodies.es_ES
dc.publisherWiley-VCH Verlag Berlines_ES
dc.subjectT helper cell determinantes_ES
dc.subjectB cell determinantes_ES
dc.subjectNeutralizing antibodieses_ES
dc.titleSimple strategy to induce antibodies of distinct specificity: application to the mapping of gp120 and inhibition of HIV-1 infectivityes_ES

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