Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Sarobe, P. (Pablo) | - |
dc.creator | Lasarte, J.J. (Juan José) | - |
dc.creator | Golvano, J.J. (José Javier) | - |
dc.creator | Prieto, I. (Isidro) | - |
dc.creator | Gullon, A. (Arturo) | - |
dc.creator | Soto, M.J. (María José) | - |
dc.creator | Labarga, P. (Pablo) | - |
dc.creator | Prieto, J. (Jesús) | - |
dc.creator | Borras-Cuesta, F. (Francisco) | - |
dc.date.accessioned | 2012-04-17T14:41:41Z | - |
dc.date.available | 2012-04-17T14:41:41Z | - |
dc.date.issued | 1994 | - |
dc.identifier.citation | Sarobe P, Lasarte JJ, Golvano JJ, Prieto I, Gullon A, Soto MJ, et al. Induction of neutralizing antibodies against human immunodeficiency virus type 1 using synthetic peptide constructs containing an immunodominant T-helper cell determinant from vpr. J Acquir Immune Defic Syndr 1994 Jul;7(7):635-640. | es_ES |
dc.identifier.issn | 0894-9255 | - |
dc.identifier.uri | https://hdl.handle.net/10171/21678 | - |
dc.description.abstract | Identification of immunodominant T-helper-cell determinants after natural infection is an important step in the design of immunogens for potential use in vaccination. Using cells from human immunodeficiency virus type 1 (HIV-1)-infected individuals and a panel of peptides encompassing the sequence of the regulatory protein vpr from HIV-1, we identified the T-helper determinant QLLFIHFRIGCRHSR, which is active in 37.5% of these individuals. To gain insight on the efficacy of this peptide in helping induce neutralizing antibodies against a B-cell determinant (BD), we synthesized constructs containing B- and T-cell determinants and tested them in BALB/c mice, the highest responders to the T-cell determinant moiety among several strains tested. These immunogens induced antibodies against two chosen B-cell determinants from HIV-1IIIB gp160 (amino acids 310-322 from the V3 loop of gp120 and 736-751 from gp41) that were able to neutralize HIV-1 infection in vitro. The highest neutralization titer against HIV-1IIIB was obtained by immunization with the homopolymer of the construct containing the T-cell epitope from vpr and the B-cell epitope from the V3 loop. We believe that the immunodominant T-cell determinant from vpr is a promising epitope to consider in the design of future peptide vaccines. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Raven Press | es_ES |
dc.rights | info:eu-repo/semantics/closedAccess | - |
dc.subject | Gene Products, vpr/immunology | es_ES |
dc.subject | HIV Antibodies/biosynthesis | es_ES |
dc.subject | HIV-1/immunology | es_ES |
dc.subject | Immunodominant Epitopes/immunology | es_ES |
dc.subject | T-Lymphocytes, Helper-Inducer/immunology | es_ES |
dc.title | Induction of neutralizing antibodies against human immunodeficiency virus type 1 using synthetic peptide constructs containing an immunodominant T-helper cell determinant from vpr | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://journals.lww.com/jaids/Abstract/1994/07000/Induction_of_Neutralizing_Antibodies_Against_Human.1.aspx | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
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