Development and validation of a liquid chromatographic method for in vitro mupirocin quantification in both skin layers and percutaneous penetration studies.
Keywords: 
Skin recovery
Mupirocin
Issue Date: 
Nov-2003
Publisher: 
Elsevier
ISSN: 
1570-0232
Citation: 
Echevarria L, Blanco-Prieto MJ, Campanero MA, Santoyo S, Ygartua P. Development and validation of a liquid chromatographic method for in vitro mupirocin quantification in both skin layers and percutaneous penetration studies. J Chromatogr B Analyt Technol Biomed Life Sci 2003 Nov 5;796(2):233-241.
Abstract
A simple, rapid, and sensitive reversed-phase high-performance liquid chromatographic (HPLC) method for the measurement of mupirocin concentrations in both skin layers and percutaneous samples has been developed. Mupirocin was extracted from skin layers using PBS–acetonitrile (90:10, v/v). The method is sufficiently sensitive and repeatable to be used in percutaneous penetration studies. The samples were chromatographed on a View the MathML source mm C8 LiChrospher Select B (5 μm). The mobile phase composition was a mixture of acetonitrile–ammonium acetate 0.05 M (27.5:72.5, v/v) adjusted to pH 6.3 with acetic acid. The analyte was detected at 228 nm and the run time was 11 min. Linearity was confirmed in the concentration range 0.2–20 μg/ml and the limit of detection was 9.5 ng/ml.

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