Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Echevarría, L. (Lydia) | - |
dc.creator | Blanco-Prieto, M.J. (María José) | - |
dc.creator | Campanero, M.A. (Miguel Angel) | - |
dc.creator | Santoyo, S. (Susana) | - |
dc.date.accessioned | 2012-06-01T09:04:31Z | - |
dc.date.available | 2012-06-01T09:04:31Z | - |
dc.date.issued | 2003-11 | - |
dc.identifier.citation | Echevarria L, Blanco-Prieto MJ, Campanero MA, Santoyo S, Ygartua P. Development and validation of a liquid chromatographic method for in vitro mupirocin quantification in both skin layers and percutaneous penetration studies. J Chromatogr B Analyt Technol Biomed Life Sci 2003 Nov 5;796(2):233-241. | es_ES |
dc.identifier.issn | 1570-0232 | - |
dc.identifier.uri | https://hdl.handle.net/10171/22388 | - |
dc.description.abstract | A simple, rapid, and sensitive reversed-phase high-performance liquid chromatographic (HPLC) method for the measurement of mupirocin concentrations in both skin layers and percutaneous samples has been developed. Mupirocin was extracted from skin layers using PBS–acetonitrile (90:10, v/v). The method is sufficiently sensitive and repeatable to be used in percutaneous penetration studies. The samples were chromatographed on a View the MathML source mm C8 LiChrospher Select B (5 μm). The mobile phase composition was a mixture of acetonitrile–ammonium acetate 0.05 M (27.5:72.5, v/v) adjusted to pH 6.3 with acetic acid. The analyte was detected at 228 nm and the run time was 11 min. Linearity was confirmed in the concentration range 0.2–20 μg/ml and the limit of detection was 9.5 ng/ml. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Skin recovery | es_ES |
dc.subject | Mupirocin | es_ES |
dc.title | Development and validation of a liquid chromatographic method for in vitro mupirocin quantification in both skin layers and percutaneous penetration studies. | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | http://dx.doi.org/10.1016/j.jchromb.2003.07.011 | es_ES |
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