Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Ansorena-Artieda, E. (Eduardo) | - |
dc.creator | Casales, E. (Erkuden) | - |
dc.creator | Aranda, A. (Alejandro) | - |
dc.creator | Tamayo, E. (Esther) | - |
dc.creator | Garbayo, E | - |
dc.creator | Smerdou, C. (Cristian) | - |
dc.creator | Blanco-Prieto, M.J. (María José) | - |
dc.creator | Aymerich-Soler, M.S. (María Soledad) | - |
dc.date.accessioned | 2012-08-24T11:19:45Z | - |
dc.date.available | 2012-08-24T11:19:45Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Ansorena E, Casales E, Aranda A, Tamayo E, Garbayo E, Smerdou C, et al. A simple and efficient method for the production of human glycosylated glial cell line-derived neurotrophic factor using a Semliki Forest virus expression system. Int J Pharm 2013 JAN 2;440(1):19-26 | es_ES |
dc.identifier.issn | 0378-5173 | - |
dc.identifier.uri | https://hdl.handle.net/10171/23071 | - |
dc.description.abstract | Human glial cell line-derived neurotrophic factor (hGDNF) is a very promising protein for the treatment of Parkinson's disease and other neurodegenerative disorders. The present work describes a quick and simple method to obtain a high amount of purified hGDNF using a mammalian cell-derived system. The method is based on the high expression level provided by a Semliki Forest virus vector and its ability to induce a strong shut-off of host-cell protein synthesis in mammalian cells. As a result, hGDNF is the only protein present in the supernatant and can be efficiently purified by a single chromatographic step. Using this system it was possible to eliminate other secreted proteins from the culture medium, like insulin-like growth factor-5, which are hard to remove using other hGDNF production methods. Purified hGDNF presents a complex glycosylation pattern typical of mammalian expression systems and is biologically active. This protocol could be extended to other secreted proteins and could be easily scaled up for industrial purposes. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | GDNF | es_ES |
dc.subject | SFV expression system | es_ES |
dc.subject | Protein expression | es_ES |
dc.subject | Protein purification | es_ES |
dc.subject | BHK cells | es_ES |
dc.subject | Glycosylated recombinant protein | es_ES |
dc.title | A simple and efficient method for the production of human glycosylated glial cell line-derived neurotrophic factor using a Semliki Forest virus expression system | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | http://dx.doi.org/10.1016/j.ijpharm.2012.04.071 | es_ES |
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