Full metadata record
DC Field | Value | Language |
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dc.creator | Molnar, C. (Clemens) | - |
dc.creator | Ruiz Garcia-Trevijano, E. (Elena) | - |
dc.creator | Ludwiczek, O. (Othmar) | - |
dc.creator | Talabot, D. (Dominique) | - |
dc.creator | Kaser, A. (Arthur) | - |
dc.creator | Mato, J.M. (José María) | - |
dc.creator | Fritsche, G. (Gernot) | - |
dc.creator | Weiss, G. (Günter) | - |
dc.creator | Gabay, C. (Cem) | - |
dc.creator | Avila, M.A. (Matías Antonio) | - |
dc.creator | Tilg, H. (Herbert) | - |
dc.date.accessioned | 2012-09-28T07:39:20Z | - |
dc.date.available | 2012-09-28T07:39:20Z | - |
dc.date.issued | 2004 | - |
dc.identifier.citation | Molnar C, Garcia-Trevijano ER, Ludwiczek O, Talabot D, Kaser A, Mato JM, et al. Anti-inflammatory effects of hepatocyte growth factor: induction of interleukin-1 receptor antagonist. Eur Cytokine Netw 2004 Oct-Dec;15(4):303-311. | es_ES |
dc.identifier.issn | 1148-5493 | - |
dc.identifier.uri | https://hdl.handle.net/10171/23250 | - |
dc.description.abstract | Hepatocyte growth factor (HGF) prevents liver failure in various animal models including endotoxin-induced acute liver failure. We were interested to find out whether human HGF exerts anti-inflammatory effects by modulation of cytokine synthesis. Therefore, human HepG2 cells were cultured with increasing concentrations of HGF. HGF dose-dependently upregulated the production of interleukin-1 receptor antagonist (IL-1Ra). Incubation of HepG2 cells with interleukin-1beta (IL-1beta) caused an increase in IL-1Ra levels, while interleukin-6 (IL-6) had no effect on IL-1Ra synthesis. Co-stimulation of HepG2 cells with HGF + IL-1beta resulted in a synergistic effect on IL-1Ra mRNA and protein expression. Stimulation of freshly isolated mouse hepatocytes from male C57 BL/6 mice with HGF increased IL-1Ra mRNA and protein synthesis dose-dependently. A co-stimulation with HGF and IL-1beta had a synergistic effect on IL-1Ra mRNA expression but only a partially additive effect on IL-1Ra protein synthesis. HGF-induced IL-1Ra production was significantly decreased by the mitogen-activated protein kinase (MAPK) inhibitor PD98059. Accordingly, HGF stimulation specifically increased MAPK-dependent signalling pathway (p42/44). In contrast, in preactivated PBMC mRNA expression and protein synthesis of IL-1Ra, interleukin-10 (IL-10) and tumor necrosis factor-alpha (TNF-alpha) were unaffected after stimulation with HGF. In conclusion, our data suggest that HGF exerts anti-inflammatory effects by modulating the signal transduction cascade leading to increased expression of IL-1Ra, which might explain the protective and regenerative properties of this cytokine in animal models of liver failure. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | John Libbey Eurotext | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | HGF | es_ES |
dc.subject | Hepatocytes | es_ES |
dc.subject | HepG2 | es_ES |
dc.subject | Monocytes | es_ES |
dc.subject | IL-1Ra | es_ES |
dc.title | Anti-inflammatory effects of hepatocyte growth factor: induction of interleukin-1 receptor antagonist | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://www.jle.com/en/revues/bio_rech/ecn/e-docs/00/04/08/31/resume.phtml | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
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