Full metadata record
DC Field | Value | Language |
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dc.creator | Martinez-Chantar, M.L. (María Luz) | - |
dc.creator | Latasa, M.U. (María Ujué) | - |
dc.creator | Varela-Rey, M. (Marta) | - |
dc.creator | Lu, S.C. (Shelly C.) | - |
dc.creator | Ruiz Garcia-Trevijano, E. (Elena) | - |
dc.creator | Mato, J.M. (José María) | - |
dc.creator | Avila, M.A. (Matías Antonio) | - |
dc.date.accessioned | 2012-10-02T17:12:20Z | - |
dc.date.available | 2012-10-02T17:12:20Z | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | Martinez-Chantar ML, Latasa MU, Varela-Rey M, Lu SC, Garcia-Trevijano ER, Mato JM, et al. L-methionine availability regulates expression of the methionine adenosyltransferase 2A gene in human hepatocarcinoma cells: role of S-adenosylmethionine. J Biol Chem 2003 May 30;278(22):19885-19890. | es_ES |
dc.identifier.issn | 1083-351X | - |
dc.identifier.uri | https://hdl.handle.net/10171/23278 | - |
dc.description.abstract | In mammals, methionine adenosyltransferase (MAT), the enzyme responsible for S-adenosylmethionine (AdoMet) synthesis, is encoded by two genes, MAT1A and MAT2A. In liver, MAT1A expression is associated with high AdoMet levels and a differentiated phenotype, whereas MAT2A expression is associated with lower AdoMet levels and a dedifferentiated phenotype. In the current study, we examined regulation of MAT2A gene expression by l-methionine availability using HepG2 cells. In l-methionine-deficient cells, MAT2A gene expression is rapidly induced, and methionine adenosyltransferase activity is increased. Restoration of l-methionine rapidly down-regulates MAT2A mRNA levels; for this effect, l-methionine needs to be converted into AdoMet. This novel action of AdoMet is not mediated through a methyl transfer reaction. MAT2A gene expression was also regulated by 5'-methylthioadenosine, but this was dependent on 5'-methylthioadenosine conversion to methionine through the salvage pathway. The transcription rate of the MAT2A gene remained unchanged during l-methionine starvation; however, its mRNA half-life was significantly increased (from 100 min to more than 3 h). The effect of l-methionine withdrawal on MAT2A mRNA stabilization requires both gene transcription and protein synthesis. We conclude that MAT2A gene expression is modulated as an adaptive response of the cell to l-methionine availability through its conversion to AdoMet. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Society for Biochemistry and Molecular Biology | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Carcinoma, Hepatocellular/metabolism | es_ES |
dc.subject | Liver Neoplasms/metabolism | es_ES |
dc.subject | Methionine/metabolism | es_ES |
dc.subject | Methionine Adenosyltransferase/genetics | es_ES |
dc.subject | S-Adenosylmethionine/physiology | es_ES |
dc.title | L-methionine availability regulates expression of the methionine adenosyltransferase 2A gene in human hepatocarcinoma cells: role of S-adenosylmethionine | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://www.jbc.org/content/278/22/19885 | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
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