Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Palop-Cubillo, J.A. (Juan Antonio) | - |
dc.contributor.advisor | Encío, I. (Ignacio) | - |
dc.creator | Lamberto, I. (Iranzu) | - |
dc.date.accessioned | 2013-04-04T08:21:15Z | - |
dc.date.available | 2013-04-04T08:21:15Z | - |
dc.date.issued | 2013-04-04 | - |
dc.date.submitted | 2012-10-24 | - |
dc.identifier.citation | LAMBERTO PÉREZ, Iranzu. “Caracterización del proceso de muerte celular inducido por los derivados bisacilimidoselenocarbamato”. Palop Cubillo, Juan Antonio; Encío Martínez, Ignacio José. Tesis doctoral. Universidad de Navarra, 2012 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10171/28202 | - |
dc.description.abstract | Bisacylimidoselenocarbamate derivatives (BSC) are potent anticancer agents with a strong cytotoxic activity against different types of tumour cells. Based in phosphatidylserine exposure on the cell membranes we show that BSC treatment resulted in enhanced cell death in leukaemia CCRF-CEM cells. DNA fragmentation detection in breast adenocarcinoma MCF-7 cells showed that BSC triggered cell death is concentration and time dependent. We also show that two of these compounds, BSC 3g and 3n, cause cell-cycle arrest in the late G2/M in MCF-7 cells. Consistent with this, a reduction in CDK1 and CDK2 expression with no change in cyclin A and B1 was observed in this cell line. Activation of caspase-2 was also detected. However, the involvement of the caspase-dependent pathway in the process of cell death induced by either BSC 3g or 3n is discarded since cell death could not be prevented by pretreatment with the pancaspase inhibitor z-VAD-fmk. Moreover, since reduced levels of p21CIP and Chk2 proteins but no change in p53 levels could be detected in MCF-7 cells after BSC 3g or 3n treatment our results suggest that BSC treated cells die from lethal mitosis. | es_ES |
dc.language.iso | spa | es_ES |
dc.publisher | Universidad de Navarra | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Materias Investigacion::Ciencias de la vida::Bioquímica | es_ES |
dc.subject | Cultivo celular | es_ES |
dc.subject | Biología molecular | es_ES |
dc.subject | Química orgánica | es_ES |
dc.title | Caracterización del proceso de muerte celular inducido por los derivados bisacilimidoselenocarbamato | es_ES |
dc.type | info:eu-repo/semantics/doctoralThesis | es_ES |
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