Full metadata record
DC FieldValueLanguage
dc.creatorDiaz-Aparicio, E. (E.)-
dc.creatorAragon, V. (V.)-
dc.creatorMarin, C.M. (C. M.)-
dc.creatorAlonso-Urmeneta, B. (Begoña)-
dc.creatorFont, M. (M.)-
dc.creatorMoreno, E. (Edgardo)-
dc.creatorPerez-Ortiz, S. (S.)-
dc.creatorBlasco, J.M. (J. M.)-
dc.creatorDiaz, R. (Ramón)-
dc.creatorMoriyon, I. (Ignacio)-
dc.identifier.citationDiaz-Aparicio E, Aragon V, Marin C, Alonso B, Font M, Moreno E, et al. Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats. J Clin Microbiol 1993 Dec;31(12):3136-3141.es_ES
dc.description.abstractHapten polysaccharides of Brucella smooth M and A serotypes were prepared from Brucella sp. and Yersinia enterocolitica O:9 by previously described hydrolytic (O chain) or nonhydrolytic (native hapten [NH]) procedures. The purified polysaccharides differed only in the presence (O chain) or absence (NH) of lipopolysaccharide core sugars. The polysaccharides were compared by reverse radial immunodiffusion for the diagnosis of brucellosis in cattle (Brucella abortus biotype 1 [A serotype] and Brucella melitensis biotype 3 [AM serotype]), sheep (B. melitensis biotypes 1 [M serotype] and 3), and goats (B. melitensis biotype 1). The reverse radial immunodiffusion test with the NH from B. melitensis 16 M (serotype M) showed the highest sensitivity (89.6 to 97.3%), regardless of the host species and the serotype of the infecting Brucella sp. Y. enterocolitica O:9 NH (A serotype) was useful for diagnosing disease in cattle infected with B. abortus biotype 1, but not in cattle infected with B. melitensis biotype 3, sheep, or goats. The different results obtained with the serotype M and A polysaccharides and the sera from animals infected with M, A, and AM serotypes of Brucella spp. showed that in naturally infected animals, a large proportion of the antibodies are directed to or react with a previously defined common epitope(s) (J. T. Douglas and D. A. Palmer, J. Clin. Microbiol. 26:1353-1356, 1988) different from the A or M epitopes. By using the radial immunodiffusion test with B. melitensis 16M NH, it was possible to differentiate infected from vaccinated cattle, sheep, and goats with a sensitivity and specificity similar to that of the complement fixation test.es_ES
dc.publisherAmerican Society for Microbiologyes_ES
dc.subjectRadial immunodiffusion testes_ES
dc.subjectLinked immunosorbent-assayes_ES
dc.subjectAgar-gel immunodifussiones_ES
dc.subjectBovine brucellosises_ES
dc.subjectNative haptenes_ES
dc.titleComparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goatses_ES

Files in This Item:
Comparative analysis.pdf
1.19 MB
Adobe PDF

Statistics and impact
0 citas en
0 citas en

Items in Dadun are protected by copyright, with all rights reserved, unless otherwise indicated.