Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Kumar, A. (Anujith) | - |
dc.creator | Lo-Nigro, A. (Antonio) | - |
dc.creator | Gysemans, C. (Conny) | - |
dc.creator | Cai, Q. (Qing) | - |
dc.creator | Esguerra, C. (Camila) | - |
dc.creator | Nelson-Holte, M. (Molly) | - |
dc.creator | Heremans, Y. (Y.) | - |
dc.creator | Jimenez-Gonzalez, M. (María) | - |
dc.creator | Porciuncula, A. (Angelo) | - |
dc.creator | Mathieu, C. (Chantal) | - |
dc.creator | Binas, B. (Bert) | - |
dc.creator | Heimberg, H. (Harry) | - |
dc.creator | Prosper-Cardoso, F. (Felipe) | - |
dc.creator | Hering, B. (Bernhard) | - |
dc.creator | Verfaillie, C.M. (Catherine M.) | - |
dc.creator | Barajas, M. (Miguel) | - |
dc.date.accessioned | 2014-08-26T12:42:14Z | - |
dc.date.available | 2014-08-26T12:42:14Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Kumar A, Lo Nigro A, Gysemans C, Cai Q, Esguerra C, Nelson-Holte M, et al. Reversal of hyperglycemia by insulin-secreting rat bone marrow- and blastocyst-derived hypoblast stem cell-like cells. PLoS One. 2013 May 9;8(5):e63491 | es_ES |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://hdl.handle.net/10171/36402 | - |
dc.description.abstract | β-cell replacement may efficiently cure type 1 diabetic (T1D) patients whose insulin-secreting β-cells have been selectively destroyed by autoantigen-reactive T cells. To generate insulin-secreting cells we used two cell sources: rat multipotent adult progenitor cells (rMAPC) and the highly similar rat extra-embryonic endoderm precursor (rXEN-P) cells isolated under rMAPC conditions from blastocysts (rHypoSC). rMAPC/rHypoSC were sequentially committed to definitive endoderm, pancreatic endoderm, and β-cell like cells. On day 21, 20% of rMAPC/rHypoSC progeny expressed Pdx1 and C-peptide. rMAPCr/HypoSC progeny secreted C-peptide under the stimulus of insulin agonist carbachol, and was inhibited by the L-type voltage-dependent calcium channel blocker nifedipine. When rMAPC or rHypoSC differentiated d21 progeny were grafted under the kidney capsule of streptozotocin-induced diabetic nude mice, hyperglycemia reversed after 4 weeks in 6/10 rMAPC- and 5/10 rHypoSC-transplanted mice. Hyperglycemia recurred within 24 hours of graft removal and the histological analysis of the retrieved grafts revealed presence of Pdx1-, Nkx6.1- and C-peptide-positive cells. The ability of both rMAPC and HypoSC to differentiate to functional β-cell like cells may serve to gain insight into signals that govern β-cell differentiation and aid in developing culture systems to commit other (pluripotent) stem cells to clinically useful β-cells for cell therapy of T1D. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library of Science | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/241447 | - |
dc.relation | info:eu-repo/grantAgreement/NIH/FP7/1U19DK061244-01 | - |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Diabetes mellitus | es_ES |
dc.subject | Embryonic stem cells | es_ES |
dc.subject | Multipotent stem cells | es_ES |
dc.subject | Hyperglycemia | es_ES |
dc.subject | Bone marrow cells | es_ES |
dc.subject | Blastocyst | es_ES |
dc.title | Reversal of hyperglycemia by insulin-secreting rat bone marrow- and blastocyst-derived hypoblast stem cell-like cells | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | http://dx.doi.org/10.1371/journal.pone.0063491 | es_ES |
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