Generation and characterization of human iPSC line generated from mesenchymal stem cells derived from adipose tissue

Rodriguez-Madoz, J.R. (Juan Roberto); Prosper-Cardoso, F. (Felipe); Barajas, M. (Miguel); Andreu, E.J. (Enrique José); Abizanda-Sarasa, G. (Gloria); Mazo, M. (Manuel); Rodriguez, S. (Saray); Zapata-Linares, N.M. (Natalia María)

Authors:

Rodriguez-Madoz, J.R. (Juan Roberto)

Prosper-Cardoso, F. (Felipe)

Barajas, M. (Miguel)

Andreu, E.J. (Enrique José)

Abizanda-Sarasa, G. (Gloria)

Mazo, M. (Manuel)

Rodriguez, S. (Saray)

Zapata-Linares, N.M. (Natalia María)

Keywords:

Materias Investigacion::Ciencias de la Salud::Microbiología y biología molecular
Materias Investigacion::Ciencias de la Salud::Oncología
Mesenchymal stem cells
Adipose tissue
Human iPSC line
Stem cell

Issue Date:

2016

Publisher:

Elsevier

Project:

the Fundación Bancaria Caja Navarra (70270).
The “Torres Quevedo” Subprogram, Ministry of Economy and Competitiveness (PTQ-11-04777)
The Genomics Core Facility of the CIMA, University of Navarra.
The Institute of Health Carlos III (PI13/00862), and RETIC (RD12/0019/0031)

ISSN:

1873-5061

Note:

An open access article under the CC BY-NC-ND license

Citation:

Zapata-Linares NM, Rodríguez S, Mazo M, Abizanda G, Andreu EJ, Barajas M, et al. Generation and characterization of human iPSC line generated from mesenchymal stem cells derived from adipose tissue. Stem Cell Res 2016;16(1):20–23

Abstract

Abstract In this work, mesenchymal stem cells derived from adipose tissue (ADSCs) were used for the generation of the human-induced pluripotent stem cell line G15.AO. Cell reprogramming was performed using retroviral vectors containing the Yamanaka factors, and the generated G15.AO hiPSC line showed normal karyotype, silencing of the exogenous reprogramming factors, induction of the typical pluripotency-associated markers, alkaline phosphatase enzymatic activity, and in vivo and in vitro differentiation ability to the three germ layers.

URI:

https://hdl.handle.net/10171/39951

DOI:

http://dx.doi.org/10.1016/j.scr.2015.12.002

Appears in Collections:

DA - CIMA - Medicina regenerativa - Terapia celular - Artículos de Revista
DA - CIMA - Terapia génica - Terapia génica del cáncer - Artículos de revista
DA - CUN - Área de Terapia Celular - Artículos de revista

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