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dc.creatorPignataro, D. (Diego)-
dc.creatorSucunza, D. (Diego)-
dc.creatorVanrell, L. (Lucía)-
dc.creatorLopez-Franco, E. (Esperanza)-
dc.creatorDopeso-Reyes, I.G. (Iria G.)-
dc.creatorVales, A. (África)-
dc.creatorHommel, M. (Mirja)-
dc.creatorRico, A.J. (Alberto J.)-
dc.creatorLanciego, J.L. (José Luis)-
dc.creatorGonzalez-Aseguinolaza, G. (Gloria)-
dc.date.accessioned2017-04-26T09:47:24Z-
dc.date.available2017-04-26T09:47:24Z-
dc.date.issued2017-
dc.identifier.citationPignataro D, Sucunza D, Vanrell L, Lopez-Franco E, Dopeso-Reyes IG, Vales A, et al. Adeno-associated viral vectors serotype 8 for cell-specific delivery of therapeutic genes in the central nervous system. Front Neuroanat 2017 Feb;11:2.es_ES
dc.identifier.issn1662-5129-
dc.identifier.urihttps://hdl.handle.net/10171/43258-
dc.description.abstractAdeno-associated viruses (AAVs) have become highly promising tools for research and clinical applications in the central nervous system (CNS). However, specific delivery of genes to the cell type of interest is essential for the success of gene therapy and therefore a correct selection of the promoter plays a very important role. Here, AAV8 vectors carrying enhanced green fluorescent protein (eGFP) as reporter gene under the transcriptional control of different CNS-specific promoters were used and compared with a strong ubiquitous promoter. Since one of the main limitations of AAV-mediated gene delivery lies in its restricted cloning capacity, we focused our work on small-sized promoters. We tested the transduction efficacy and specificity of each vector after stereotactic injection into the mouse striatum. Three glia-specific AAV vectors were generated using two truncated forms of the human promoter for glial fibrillar acidic protein (GFAP) as well as a truncated form of the murine GFAP promoter. All three vectors resulted in predominantly glial expression; however we also observed eGFP expression in other cell-types such as oligodendrocytes, but never in neurons. In addition, robust and neuron-specific eGFP expression was observed using the minimal promoters for the neural protein BM88 and the neuronal nicotinic receptor β2 (CHRNB2). In summary, we developed a set of AAV vectors designed for specific expression in cells of the CNS using minimal promoters to drive gene expression when the size of the therapeutic gene matters.es_ES
dc.language.isospaes_ES
dc.publisherFrontiers Mediaes_ES
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/340527;286071-
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectMaterias Investigacion::Ciencias de la Saludes_ES
dc.subjectAAVes_ES
dc.subjectCNSes_ES
dc.subjectPromoterses_ES
dc.subjectBasal gangliaes_ES
dc.subjectParkinson's diseasees_ES
dc.subjectGene therapyes_ES
dc.titleAdeno-associated viral vectors serotype 8 for cell-specific delivery of therapeutic genes in the central nervous systemes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.noteCC-BY license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and the source are credited.es_ES
dc.editorial.noteThis Document is Protected by copyright and was first published by Frontiers. All rights reserved. it is reproduced with permission.es_ES
dc.identifier.doihttp://doi.org/10.3389/fnana.2017.00002es_ES

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