Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Rodríguez-Losada, N. (Noela) | - |
dc.creator | Rosa-Fernández-Pacheco, F.J. (Francisco Javier) de la | - |
dc.creator | Larriva-Hormigos, M. (María) | - |
dc.creator | Wendelbo, R. (Rune) | - |
dc.creator | Aguirre, J.A. (José A.) | - |
dc.creator | Saez-Castresana, J. (Javier) | - |
dc.creator | Ballaz, S.J. (Santiago J.) | - |
dc.date.accessioned | 2020-10-29T14:25:44Z | - |
dc.date.available | 2020-10-29T14:25:44Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Rodríguez-Losada, N. (Noela); Rosa-Fernández-Pacheco, F.J. (Francisco Javier) de la; Larriva-Hormigos, M. (María); et al. "Overexpression of alpha-synuclein promotes both cell proliferation and cell toxicity in human SH-SY5Y neuroblastoma cells". Journal of Advanced Research. 23, 2020, 37 - 45 | es |
dc.identifier.issn | 2090-1232 | - |
dc.identifier.uri | https://hdl.handle.net/10171/59584 | - |
dc.description.abstract | Alpha-Synuclein (aSyn) is a chameleon-like protein. Its overexpression and intracellular deposition defines neurodegenerative alpha-synucleinopathies including Parkinson's disease. Whether aSyn upregulation is the cause or the protective reaction to alpha-synucleinopathies remains unresolved. Remarkably, the accumulation of aSyn is involved in cancer. Here, the neuroblastoma SH-SY5Y cell line was genetically engineered to overexpress aSyn at low and at high levels. aSyn cytotoxicity was assessed by the MIT and vital-dye exclusion methods, observed at the beginning of the sub-culture of low-aSyn overexpressing neurons when cells can barely proliferate exponentially. Conversely, high-aSyn overexpressing cultures grew at high rates while showing enhanced colony formation compared to low-aSyn neurons. Cytotoxicity of aSyn overexpression was indirectly revealed by the addition of pro-oxidant rotenone. Pretreatment with partially reduced graphene oxide, an apoptotic agent, increased toxicity of rotenone in low-aSyn neurons, but, it did not in high-aSyn neurons. Consistent with their enhanced proliferation, high-aSyn neurons showed elevated levels of SMP30, a senescence-marker protein, and the mitosis Ki-67 marker. | - |
dc.language.iso | en | - |
dc.rights | info:eu-repo/semantics/openAccess | - |
dc.subject | Alpha-synuclein | - |
dc.subject | SH-SY5Y cells | - |
dc.subject | Rotenone | - |
dc.subject | Graphene oxide | - |
dc.subject | Parkinson's disease | - |
dc.subject | Cell senescence | - |
dc.title | Overexpression of alpha-synuclein promotes both cell proliferation and cell toxicity in human SH-SY5Y neuroblastoma cells | - |
dc.type | info:eu-repo/semantics/article | - |
dc.description.note | CC BY NC ND | - |
dc.identifier.doi | 10.1016/j.jare.2020.01.009 | - |
dadun.citation.endingPage | 45 | - |
dadun.citation.publicationName | Journal of Advanced Research | - |
dadun.citation.startingPage | 37 | - |
dadun.citation.volume | 23 | - |
Files in This Item:
Statistics and impact
Items in Dadun are protected by copyright, with all rights reserved, unless otherwise indicated.