Endothelial Nox5 expression modulates glucose uptake and lipid accumulation in mice fed a high-fat diet and 3T3-L1 adipocytes treated with glucose and palmitic acid
Keywords: 
Obesity
NADPH oxidase 5
Lipid homeostasis
Glucose uptake
Caveolin 1
Glut4
Issue Date: 
2021
ISSN: 
1422-0067
Note: 
This article is an open accessarticle distributed under the terms and conditions of the Creative Commons Attribution
Citation: 
García-García, J. (Jorge); Ansorena-Artieda, E. (Eduardo); Milagro-Yoldi, F.I. (Fermín Ignacio); et al. "Endothelial Nox5 expression modulates glucose uptake and lipid accumulation in mice fed a high-fat diet and 3T3-L1 adipocytes treated with glucose and palmitic acid". International Journal of Molecular Sciences. 22 (5), 2021, 2729
Abstract
Obesity is a global health issue associated with insulin resistance and altered lipid homeostasis. It has been described that reactive oxygen species (ROS) derived from nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) activity are involved in the development of these pathologies. The present study describes the role of endothelial NOX5 expression over adipose tissue by using two experimental systems: NOX5 conditional knock-in mice fed with a high-fat diet and 3T3-L1 adipocytes cultured with conditioned media of NOX5-expressing endothelial cells previously treated with glucose and palmitic acid. Animals expressing NOX5 presented lower body weight gain and less mesenteric and epididymal adipose mass compared to control mice fed with the same diet. NOX5-expressing mice also showed significantly lower glycaemia and improved insulin-induced glucose uptake. In addition, Glut4 and Caveolin 1 (Cav1) expression were significantly increased in the adipose tissue of these animals. Likewise, 3T3-L1 adipocytes treated with conditioned media from NOX5-expressing endothelial cells, incubated with high glucose and palmitic acid, presented a reduction in lipid accumulation and an increase in glucose uptake. Moreover, a significant increase in the expression of Glut4 and Cav1 was also detected in these cells. Taken together, all these data support that, in response to a highly caloric diet, NOX5 endothelial activity may regulate glucose sensitivity and lipid homeostasis in the adipose tissue.

Files in This Item:
Thumbnail
File
pdf.pdf
Description
Size
11.15 MB
Format
Adobe PDF


Statistics and impact

Items in Dadun are protected by copyright, with all rights reserved, unless otherwise indicated.