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dc.creatorGuzman-Verri, C. (Caterina)-
dc.creatorSuárez-Esquivel, M. (Marcela)-
dc.creatorRuíz-Villalobos, N. (Nazareth)-
dc.creatorZygmunt, M. (Michel)-
dc.creatorGonnet, M. (Mathieu)-
dc.creatorCampos, E. (Elena)-
dc.creatorVíquez-Ruiz, E. (Eunice)-
dc.creatorChacon-Diaz, C. (Carlos)-
dc.creatorAragón-Aranda, B. (Beatriz)-
dc.creatorRaquel-
dc.creatorMoriyon, I. (Ignacio)-
dc.creatorBlasco, J.M. (J. M.)-
dc.creatorMuñoz, P. (Pilar)-
dc.creatorBaker, K.S. (Kate S.)-
dc.creatorThomson, N.R. (Nicholas R.)-
dc.creatorCloeckaert, A. (Axel)-
dc.creatorMoreno, E. (Edgardo)-
dc.date.accessioned2021-08-24T11:24:09Z-
dc.date.available2021-08-24T11:24:09Z-
dc.date.issued2019-
dc.identifier.citationGuzman-Verri, C. (Caterina); Suárez-Esquivel, M. (Marcela); Ruíz-Villalobos, N. (Nazareth); et al. "Genetic and Phenotypic Characterization of the Etiological Agent of Canine Orchiepididymitis Smooth Brucella sp. BCCN84.3". Frontiers in Veterinary Science. 6 (175), 2019, 1 - 13es_ES
dc.identifier.issn2297-1769-
dc.identifier.urihttps://hdl.handle.net/10171/61572-
dc.description.abstractMembers of the genus Brucella cluster in two phylogenetic groups: classical and non-classical species. The former group is composed of Brucella species that cause disease in mammals, including humans. A Brucella species, labeled as Brucella sp. BCCN84.3, was isolated from the testes of a Saint Bernard dog suffering orchiepididymitis, in Costa Rica. Following standard microbiological methods, the bacterium was first defined as “Brucella melitensis biovar 2.” Further molecular typing, identified the strain as an atypical “Brucella suis.” Distinctive Brucella sp. BCCN84.3 markers, absent in other Brucella species and strains, were revealed by fatty acid methyl ester analysis, high resolution melting PCR and omp25 and omp2a/omp2b gene diversity. Analysis of multiple loci variable number of tandem repeats and whole genome sequencing demonstrated that this isolate was different from the currently described Brucella species. The smooth Brucella sp. BCCN84.3 clusters together with the classical Brucella clade and displays all the genes required for virulence. Brucella sp. BCCN84.3 is a species nova taxonomical entity displaying pathogenicity; therefore, relevant for differential diagnoses in the context of brucellosis. Considering the debate on the Brucella species concept, there is a need to describe the extant taxonomical entities of these pathogens in order to understand the dispersion and evolution.es_ES
dc.description.sponsorshipThis work was supported by FEES-CONARE, Costa Rica; Fondo Institucional de Desarrollo Académico (FIDA), Universidad Nacional; Wellcome Trust; CITA-INIA, Spain (project Bru-Epidia 291815-FP7/ERANET/ANIHWA); MINECO (AGL2014-58795-CA), and Aragon Government (Consolidated Group A14). Authors from the Sanger Institute were supported by Wellcome Trust (098051). NR-V was partially sponsored by a scholarship from the University of Costa Rica. KB was founded by a Wellcome Trust Postdoctoral Training Fellowship for Clinicians (106690/Z/14/Z). MS-E was granted with a fellowship from SEP, Universidad de Costa Rica.es_ES
dc.language.isoenges_ES
dc.publisherFrontiers Media SAes_ES
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/291815/EU//es_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectMaterias Investigacion::Ciencias de la vida::Genéticaes_ES
dc.subjectBrucellaes_ES
dc.subjectBrucella melitensises_ES
dc.subjectBrucella suises_ES
dc.subjectBrucella canises_ES
dc.subjectBrucellosises_ES
dc.subjectDoges_ES
dc.subjectSpecieses_ES
dc.subjectEpididymitises_ES
dc.titleGenetic and Phenotypic Characterization of the Etiological Agent of Canine Orchiepididymitis Smooth Brucella sp. BCCN84.3es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.noteFrontiers is fully compliant with open access mandates, by publishing its articles under the Creative Commons Attribution licence (CC-BY)es_ES
dc.identifier.doi10.3389/fvets.2019.00175-
dadun.citation.endingPage13es_ES
dadun.citation.number175es_ES
dadun.citation.publicationNameFrontiers in Veterinary Sciencees_ES
dadun.citation.startingPage1es_ES
dadun.citation.volume6es_ES

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