Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Andrea, C.E. (Carlos Eduardo) de | - |
dc.creator | Ochoa, M.C. (María Carmen) | - |
dc.creator | Villalba-Esparza, M. (María) | - |
dc.creator | Teijeira, A. (Álvaro) | - |
dc.creator | Schalper, K.A. (Kurt A.) | - |
dc.creator | Abengozar-Muela, M. (Marta) | - |
dc.creator | Eguren-Santamaría, I. (Iñaki) | - |
dc.creator | Sainz, C. (Cristina) | - |
dc.creator | Sánchez-Gregorio, S. (Sandra) | - |
dc.creator | Garasa, S. (Saray) | - |
dc.creator | Ariz, M. (Mikel) | - |
dc.creator | Ortiz-de-Solorzano, C. (Carlos) | - |
dc.creator | Rodriguez-Ruiz, M.E. (María Esperanza) | - |
dc.creator | Perez-Gracia, J.L. (Jose Luis) | - |
dc.creator | Lozano, M.D. (María Dolores) | - |
dc.creator | Echeveste, J.I. (José I.) | - |
dc.creator | Fernandez-Sanmamed, M. (Miguel) | - |
dc.creator | Melero, I. (Ignacio) | - |
dc.date.accessioned | 2021-09-17T09:36:34Z | - |
dc.date.available | 2021-09-17T09:36:34Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Andrea, C.E. (Carlos Eduardo) de; Ochoa, M.C. (María Carmen); Villalba-Esparza, M. (María); et al. "Heterogenous presence of neutrophil extracellular traps in human solid tumours is partially dependent on IL-8". The Journal of Pathology. 255 (2), 2021, 190 - 201 | es |
dc.identifier.issn | 1096-9896 | - |
dc.identifier.uri | https://hdl.handle.net/10171/62004 | - |
dc.description.abstract | Neutrophil extracellular traps (NETs) are webs of extracellular nuclear DNA extruded by dying neutrophils infiltrating tissue. NETs constitute a defence mechanism to entrap and kill fungi and bacteria. Tumours induce the formation of NETs to the advantage of the malignancy via a variety of mechanisms shown in mouse models. Here, we investigated the presence of NETs in a variety of human solid tumours and their association with IL-8 (CXCL8) protein expression and CD8+ T-cell density in the tumour microenvironment. Multiplex immunofluorescence panels were developed to identify NETs in human cancer tissues by co-staining with the granulocyte marker CD15, the neutrophil marker myeloperoxidase and citrullinated histone H3 (H3Cit), as well as IL-8 protein and CD8+ T cells. Three ELISA methods to detect and quantify circulating NETs in serum were optimised and utilised. Whole tumour sections and tissue microarrays from patients with non-small cell lung cancer (NSCLC; n = 14), bladder cancer (n = 14), melanoma (n = 11), breast cancer (n = 31), colorectal cancer (n = 20) and mesothelioma (n = 61) were studied. Also, serum samples collected retrospectively from patients with metastatic melanoma (n = 12) and NSCLC (n = 34) were ELISA assayed to quantify circulating NETs and IL-8. NETs were detected in six different human cancer types with wide individual variation in terms of tissue density and distribution. At least in NSCLC, bladder cancer and metastatic melanoma, NET density positively correlated with IL-8 protein expression and inversely correlated with CD8+ T-cell densities. In a series of serum samples from melanoma and NSCLC patients, a positive correlation between circulating NETs and IL-8 was found. In conclusion, NETs are detectable in formalin-fixed human biopsy samples from solid tumours and in the circulation of cancer patients with a considerable degree of individual variation. NETs show a positive association with IL-8 and a trend towards a negative association with CD8+ tumour-infiltrating lymphocytes. | es_ES |
dc.description.sponsorship | This project has been supported by grants from the I-ON program from Bristol-Myers Squibb, Partners of Choice Network from AstraZeneca, MINECO SAF2017-83267-C2-1-R (AEI/FEDER, UE) and Instituto de Salud Carlos III and co-financed by Fondos FEDER. This project has also received funding from the European Union’s Horizon 2020 Research and Innovation Program (grant agreement no. 635122 - PROCROP), Fundaci on de la Asociaci on Española Contra el Cancer (AECC) and Cancer Research UK (Accelerator Project: Local Radioimmunotherapy). AT has received financial support through ‘la Caixa’ Banking Foundation (LCF/BQ/LR18/11640014). COS has received financial support through the Spanish Ministry of Science, Innovation and Universities, under grants RTI2018-094494-BC22 and RTC-2017-6218-1 (MCIU/AEI/FEDER, UE). MFS is supported by a Miguel Servet contract from Instituto de Salud Carlos III, Fondo de Investigacion Sanitaria (Spain). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/635122/EU | - |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | NETs | es_ES |
dc.subject | Neutrophil extracellular traps | es_ES |
dc.subject | Neutrophils forming NETs | es_ES |
dc.subject | Neutrophils | es_ES |
dc.subject | IL-8 | es_ES |
dc.subject | CXCR1/CXCR2 | es_ES |
dc.subject | Solid tumour | es_ES |
dc.title | Heterogenous presence of neutrophil extracellular traps in human solid tumours is partially dependent on IL-8 | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.description.note | This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License | es_ES |
dc.identifier.doi | 10.1002/path.5753 | - |
dadun.citation.endingPage | 201 | es_ES |
dadun.citation.number | 2 | es_ES |
dadun.citation.publicationName | The Journal of Pathology | es_ES |
dadun.citation.startingPage | 190 | es_ES |
dadun.citation.volume | 255 | es_ES |
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