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dc.creatorRubio-Garrido, M. (Marina)-
dc.creatorNdaraburu, A. (Adolphe)-
dc.creatorReina, G. (Gabriel)-
dc.creatorBarquín, D. (David)-
dc.creatorFernández-Alonso, M. (Miriam)-
dc.creatorCarlos, S. (Silvia)-
dc.creatorHolguín, Á. (África)-
dc.identifier.citationRubio-Garrido, M. (Marina); Ndaraburu, A. (Adolphe); Reina, G. (Gabriel); et al. "Utility Of POC Xpert HIV-1 Tests For Detection-Quantifcation Of Complex HIV Recombinants Using Dried Blood Spots From Kinshasa, D. R. Congo". Scientific Reports. 9 (5679), 2019, 1 - 11es
dc.identifier.otherPMID: 30952893-
dc.description.abstractPoint-of-Care (POC) molecular assays improve HIV infant diagnosis and viral load (VL) quantification in resource-limited settings. We evaluated POC performance in Kinshasa (Democratic Republic of Congo), with high diversity of HIV-1 recombinants. In 2016, 160 dried blood samples (DBS) were collected from 85 children (60 HIV-, 18 HIV+, 7 HIV-exposed) and 75 HIV+ adults (65 treated, 10 naive) at Monkole Hospital (Kinshasa). We compared viraemia with Cepheid-POC-Xpert-HIV-1VL and the non-POC-COBAS®AmpliPrep/COBAS®TaqMan®HIV-1-Testv2 in all HIV+, carrying 72.4%/7.2% HIV-1 unique/complex recombinant forms (URF/CRF). HIV-1 infection was confirmed in 14 HIV+ children by Cepheid-POC-Xpert-HIV-1Qual and in 70 HIV+ adults by both Xpert-VL and Roche-VL, identifying 8 false HIV+ diagnosis performed in DRC (4 adults, 4 children). HIV-1 was detected in 95.2% and 97.6% of 84 HIV+ samples by Xpert-VL and Roche-VL, respectively. Most (92.9%) HIV+ children presented detectable viraemia by both VL assays and 74.3% or 72.8% of 70 HIV+ adults by Xpert or Roche, respectively. Both VL assays presented high correlation (R2 = 0.89), but showing clinical relevant ≥0.5 log VL differences in 15.4% of 78 cases with VL within quantification range by both assays. This is the first study confirming the utility of Xpert HIV-1 tests for detection-quantification of complex recombinants currently circulating in Kinshasa.es_ES
dc.description.sponsorshipTis study was funded by fundraising and donations (mainly of Bomberos Ayudan Association), by the Government of Spain (Fondo de Investigación en Salud-FI, grants PI16/01908 and PI18/00904) and the Government of Navarre (grant 045-2015). Te study was integrated in the research supported by the Spanish HIV infected Paediatric Cohort (CoRISpe) integrated in the Spanish AIDS Research Network and by Instituto de Salud Carlos III, Spanish Health Ministry (Grant no. RD06/0025-ISCIII-FEDER). Tis study is also included in the “Subprograma de Inmigración y Salud” from CIBERESP (Spain). Xpert VL cartridges to study DBS samples from adult patients were supplied as a donation from Werfen Companyes_ES
dc.publisherSpringer Science and Business Media LLCes_ES
dc.subjectMaterias Investigacion::Ciencias de la Salud::Microbiología y biología moleculares_ES
dc.titleUtility Of POC Xpert HIV-1 Tests For Detection-Quantifcation Of Complex HIV Recombinants Using Dried Blood Spots From Kinshasa, D. R. Congoes_ES
dc.description.noteThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.es_ES
dadun.citation.publicationNameScientific Reportses_ES

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