Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Doush, Y. (Yegor) | - |
dc.creator | Surani, A.A. (Arif A.) | - |
dc.creator | Navarro-Corcuera, A. (Amaia) | - |
dc.creator | McArdle, S. (Stephanie) | - |
dc.creator | Billett, E.E. (E. Ellen) | - |
dc.creator | Montiel-Duarte, C. (Cristina) | - |
dc.date.accessioned | 2021-12-09T10:57:06Z | - |
dc.date.available | 2021-12-09T10:57:06Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Doush, Y. (Yegor); Surani, A.A. (Arif A.); Navarro-Corcuera, A. (Amaia); et al. "SP1 and RARα regulate AGAP2 expression in cancer". Scientific Reports. 9 (390), 2019, 1 - 12 | es_ES |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.other | PMID: 30674964 | - |
dc.identifier.uri | https://hdl.handle.net/10171/62621 | - |
dc.description.abstract | AGAP2 (Arf GAP with GTP-binding protein-like domain, Ankyrin repeat and PH domain 2) isoform 2 is considered a proto-oncogene, but not much is known about AGAP2 gene expression regulation. To get some insight into this process, AGAP2 proximal promoter was cloned and characterised using reporter assays. We have identified SP1 as a transcription factor bound to AGAP2 promoter and required for AGAP2 expression in two different types of cancer cells (KU812, a chronic myeloid leukaemia cell line; and DU145, a prostate cancer cell line): silencing SP1 decreased AGAP2 protein levels. We have also found that all-trans retinoic acid (ATRA) treatment increased AGAP2 protein levels in both cell lines whilst curcumin treatment reduced ATRA-mediated AGAP2 increase. Furthermore, chromatin immunoprecipitation studies revealed the presence of RARα, RXRα and the lysine acetyl transferase PCAF in AGAP2 promoter. Our results provide a novel understanding of AGAP2 expression regulation that could be beneficial to those patients with cancers where AGAP2 is overexpressed. | es_ES |
dc.description.sponsorship | Dr. Yegor Doush’s and Dr. Arif Surani’s work was supported by funds from Nottingham Trent University. Miss Amaia Navarro-Corcuera’s work was supported by a grant from the Asociacion de Amigos de la Universidad de Navarra and by a Mobility Award from Santander. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Springer Science and Business Media LLC | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Materias Investigacion::Ciencias de la Salud::Oncología | es_ES |
dc.subject | AGAP2 | es_ES |
dc.subject | Cancer | es_ES |
dc.subject | SP1 | es_ES |
dc.subject | RARα | es_ES |
dc.title | SP1 and RARα regulate AGAP2 expression in cancer | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.description.note | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made | es_ES |
dc.identifier.doi | 10.1038/s41598-018-36888-x | - |
dadun.citation.endingPage | 12 | es_ES |
dadun.citation.number | 390 | es_ES |
dadun.citation.publicationName | Scientific Reports | es_ES |
dadun.citation.startingPage | 1 | es_ES |
dadun.citation.volume | 9 | es_ES |
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