Full metadata record
DC Field | Value | Language |
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dc.creator | Gómez-de-Segura, I. (Iranzu) | - |
dc.creator | Ahechu-Garayoa, P. (Patricia) | - |
dc.creator | Gomez-Ambrosi, J. (Javier) | - |
dc.creator | Rodriguez, A. (Amaia) | - |
dc.creator | Ramirez, B. (Beatriz) | - |
dc.creator | Becerril, S. (Sara) | - |
dc.creator | Unamuno, X. (Xabier) | - |
dc.creator | Mentxaka, A. (Amaia) | - |
dc.creator | Baixauli-Fons, J. (Jorge) | - |
dc.creator | Valenti, V. (Víctor) | - |
dc.creator | Moncada, R. (Rafael) | - |
dc.creator | Silva, C. (Camilo) | - |
dc.creator | Frühbeck, G. (Gema) | - |
dc.creator | Catalan, V. (Victoria) | - |
dc.date.accessioned | 2022-01-11T09:42:37Z | - |
dc.date.available | 2022-01-11T09:42:37Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Gómez de Segura, I.; Ahechu-Garayoa, P. (Patricia); Gómez-Ambrosi, J. (Javier); et al. "Decreased Levels of Microfibril-Associated Glycoprotein (MAGP)-1 in Patients with Colon Cancer and Obesity Are Associated with Changes in Extracellular Matrix Remodelling". International Journal of Molecular Sciences. 22 (16), 2021, 8485 | es |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | https://hdl.handle.net/10171/62697 | - |
dc.description.abstract | Objective: The protein microfibril-associated glycoprotein (MAGP)-1 constitutes a crucial extracellular matrix protein. We aimed to determine its impact on visceral adipose tissue (VAT) remodelling during obesity-associated colon cancer (CC). Methods: Samples obtained from 79 subjects (29 normoponderal (NP) (17 with CC) and 50 patients with obesity (OB) (19 with CC)) were used in the study. Circulating concentrations of MAGP-1 and its gene expression levels (MFAP2) in VAT were analysed. The impact of inflammation-related factors and adipocyte-conditioned media (ACM) on MFAP2 mRNA levels in colon adenocarcinoma HT-29 cells were further analysed. The effects of MAGP-1 in the expression of genes involved in the extracellular matrix (ECM) remodelling and tumorigenesis in HT-29 cells was also explored. Results: Obesity (p < 0.01) and CC (p < 0.001) significantly decreased MFAP2 gene expression levels in VAT whereas an opposite trend in TGFB1 mRNA levels was observed. Increased mRNA levels of MFAP2 after the stimulation of HT-29 cells with lipopolysaccharide (LPS) (p < 0.01) and interleukin (IL)-4 (p < 0.01) together with a downregulation (p < 0.05) after hypoxia mimicked by CoCl2 treatment was observed. MAGP-1 treatment significantly enhanced the mRNA levels of the ECM-remodelling genes collagen type 6 alpha 3 chain (COL6A3) (p < 0.05), decorin (DCN) (p < 0.01), osteopontin (SPP1) (p < 0.05) and TGFB1 (p < 0.05). Furthermore, MAGP-1 significantly reduced (p < 0.05) the gene expression levels of prostaglandin-endoperoxide synthase 2 (COX2/PTGS2), a key gene controlling cell proliferation, growth and adhesion in CC. Interestingly, a significant decrease (p < 0.01) in the mRNA levels of MFAP2 in HT-29 cells preincubated with ACM from volunteers with obesity compared with control media was observed. Conclusion: The decreased levels of MAGP-1 in patients with obesity and CC together with its capacity to modulate key genes involved in ECM remodelling and tumorigenesis suggest MAGP-1 as a link between AT excess and obesity-associated CC development. | - |
dc.language.iso | en | - |
dc.rights | info:eu-repo/semantics/openAccess | - |
dc.subject | Obesity | - |
dc.subject | Colon cancer | - |
dc.subject | Adipose tissue | - |
dc.subject | Inflammation | - |
dc.subject | Extracellular matrix remodelling | - |
dc.title | Decreased Levels of Microfibril-Associated Glycoprotein (MAGP)-1 in Patients with Colon Cancer and Obesity Are Associated with Changes in Extracellular Matrix Remodelling | - |
dc.type | info:eu-repo/semantics/article | - |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/22/16/8485 | - |
dc.description.note | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). | - |
dc.identifier.doi | 10.3390/ijms22168485 | - |
dadun.citation.number | 16 | - |
dadun.citation.publicationName | International Journal of Molecular Sciences | - |
dadun.citation.startingPage | 8485 | - |
dadun.citation.volume | 22 | - |
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