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dc.creatorIsidoro-García, M (María)-
dc.creatorGarcía-Sánchez, A. (Asunción)-
dc.creatorSanz, C. (C.)-
dc.creatorEstravís, M. (Miguel)-
dc.creatorMarcos-Vadillo, E. (Elena)-
dc.creatorPascual, M. (Marien)-
dc.creatorRoa, S. (Sergio)-
dc.creatorMarques-García, F. (Fernando)-
dc.creatorTrivino, J.C. (Juan Carlos)-
dc.creatorDavila, I. (I.)-
dc.date.accessioned2022-02-14T08:10:33Z-
dc.date.available2022-02-14T08:10:33Z-
dc.date.issued2019-
dc.identifier.citationIsidoro-García, M (María); García-Sánchez, A. (Asunción); Sanz, C. (C.); et al. "YRNAs overexpression and potential implications in allergy". World Allergy Organization Journal. 12 (8), 2019, 100047es_ES
dc.identifier.issn1939-4551-
dc.identifier.urihttps://hdl.handle.net/10171/62911-
dc.description.abstractSmall non-coding RNAs (snRNAs) develop important functions related to epigenetic regulation. YRNAs are snRNAs involved in the initiation of DNA replication and RNA stability that regulate gene expression. They have been related to autoimmune, cancer and inflammatory diseases but never before to allergy. In this work we described for the first time in allergic patients the differential expression profile of YRNAs, their regulatory mechanisms and their potential as new diagnostic and therapeutic targets. Methods: From a previous whole RNAseq study in B cells of allergic patients, differential expression profiles of coding and non-coding transcripts were obtained. To select the most differentially expressed non coding transcripts, fold change and p-values were analyzed. A validation of the expression differences detected was developed in an independent cohort of 304 individuals, 208 allergic patients and 96 controls by using qPCR. Potential binding and retrotransponibility capacity were characterized by in silico structural analysis. Using a novel bioinformatics approach, RNA targets identification, functional enrichment and network analyses were performed. Results: We found that almost 70% of overexpressed non-coding transcripts in allergic patients corresponded to YRNAs. From the three more differentially overexpressed candidates, increased expression was independently confirmed in the peripheral blood of allergic patients. Structural analysis suggested a protein binding capacity decrease and an increase in retrotransponibility. Studies of RNA targets allowed the identification of sequences related to the immune mechanisms underlying allergy. Conclusions: Overexpression of YRNAs is observed for the first time in allergic patients. Structural and functional information points to their implication on regulatory mechanisms of the disease.es_ES
dc.description.sponsorshipThis work was supported by the Instituto de Salud Carlos III and the European Regional Development Fund (grantsPI13/00564, PI17/ 00818), and by Junta de Castilla y Leon (grants GRS1189/A/15, GRS1596/A/17) and Consolidated Research Unit of Castilla y Leon (CAS086P17). SR is funded by the Ramon y Cajal program (RYC-2014- 16399), and a grant from FEDER/Ministerio de Ciencia, Innovacion y Universidades - Agencia Estatal de Investigacion (SAF2017-82309-R). This work was partially supported by a grant of the Instituto de Salud Carlos III (ISCIII) “Red tem atica de investigacion en salud Asma, Reac- ciones Adversas y Al ergicas (ARADYAL)” RD16/0006/0019.es_ES
dc.language.isoenges_ES
dc.publisherElsevier BVes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectAllergyes_ES
dc.subjectEpigeneticses_ES
dc.subjectExpressiones_ES
dc.subjectncRNAes_ES
dc.subjectTranscriptomicses_ES
dc.subjectYRNAses_ES
dc.titleYRNAs overexpression and potential implications in allergyes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.noteThis is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).es_ES
dc.identifier.doi10.1016/j.waojou.2019.100047-
dadun.citation.number8es_ES
dadun.citation.publicationNameWorld Allergy Organization Journales_ES
dadun.citation.startingPage100047es_ES
dadun.citation.volume12es_ES

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